2020
Huang K, Marchesi A, Hollingsworth K, Both P, Mattey A, Pallister E, Ledru H, Charnock S, Galen MC, Turnbull B,Parmeggiani F and Flitsch SL. Biochemical characterisation of an α1,4 galactosyltransferase from Neisseria weaveri for the synthesis of α1,4-linked galactosides. Org. Biomol. Chem., 2020, AcceptedManuscript https://pubs.rsc.org/en/content/articlelanding/2020/ob/d0ob00407c#!divAbstract2019
2019
Laborda, P, Lyu, Y-M+, Parmeggiani F, Lu, A-M,Wang, J-W,Huang, Kun Huang, Juan Guo, J, Liu, L, Flitsch, SL, and Voglmeir, J. Enzymatic N-acylation Step Enables the Biocatalytic Synthesis of Unnatural Sialosides. Angewandte Chemie. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201914338
Cosgrove, S, Mattey A, Riese M, Chapman M, Birmingham W, Blacker AJ, Kapur N, Turner, NJ, Flitsch SL Biocatalytic Oxidation in Continuous Flow for the Generation of Carbohydrate Dialdehydes. ACS Catalysis, accepted manuscript, Manuscript ID: cs-2019-04819x.R1.
Weise NJ, Thapa P, Ahmed ST, Heath RS, Parmeggiani F, Turner NJ, Flitsch, SL, Bi-enzymatic Conversion of Cinnamic Acids to 2-Arylethylamines. ChemCatChem, https://doi.org/10.1002/cctc.201902128
Pallister E, Choo MSF, Tai L-N, Leong DSZ, Tang W-Q, Ng SK, Huang,K, Marchesi,A, Both P, Gray C, Rudd P, Flitsch SL, Nguyen-Khuong,T. Exploiting the Di-Sialyl Galactose Activity of α2,6 Sialyltransferase from Photobacterium Damselae to Generate a Highly Sialylated Recombinant Alpha-1-Antitrypsin. Biochemistry, 2019 in press. https://doi.org/10.1021/acs.biochem.9b00563
Gray JC ,Lukasz G, Migas,Barran P E.,1 Pagel K, Seeberger PH.,Eyers CE.,Boons Geert-J,Pohl NLB., Compagnon I, Widmalm G , and Flitsch S. ESI: Advancing Solutions to the Carbohydrate Sequencing Challenge. Journal of the American Chemical Society, 2019, 141, 37, 14463-14479. https://doi.org/10.1021/jacs.9b06406
Mattey AP, Birmingham WR, Both P, Kress N, Huang K, van Munster J M, Bulmer G S, Parmeggiani F,† Voglmeir J, Martinez JER, Turner N J, Flitsch S L: Selective Oxidation of N-Glycolylneuraminic Acid Using an Engineered Galactose Oxidase Variant. ACS Catalysis , 2019, 9, 9, 8208-8212. https://doi.org/10.1021/acscatal.9b02873
Huang K, Flitsch SL. Glyco-enzymatic Cascades Get Protection. Nature Catalysis, 2019, 2, 479-480. https://www.nature.com/articles/s41929-019-0294-7
Hepworth J, Flitsch S, Remote Control with Engineered Enzymes. Science, 2019, 364, 6440, 529. https://doi.org/10.1126/science.aax3335
Reprint: htp://science.sciencemag.org/cgi/rapidpdf/364/6440/529?ijkey=XTc2EXXyr/kuA&keytype=ref&siteid=sci
Gamayun I, O’Keefe S, Pick T, Klein M-C, Nguyen D, McKibbin C, Piacenti M, Williams HM, Flitsch SL, Whitehead RC, Swanton E, Helms V, High S, Zimmermann, Cavalie A Eeyarestatin Compounds Selectively Enhance Sec61-Mediated Ca2+ Leakage from the Endoplasmic Reticulum. Cell Chemical Biology, 2019, 26, 571-583. https://di.org/10.1016/j.chembiol.2019.01.010
Manning, J, Tavanti M, Porter JL, Kress N, De Visser SP, Turner NJ and Flitsch,SL. Regio- and Enantio-selective Chemo-enzymatic C-H-lactonization of Decanoic Acid to (S)-δ-Decalactone. Angewandte Chemie, 2019, 58, 5668-5671. https://doi.org/ 10.1002/anie.201901242
Keenan T, Mills R; Pocock E,Budhadev D, Parmeggiani F, Flitsch, SL, Fascione, M. The characterisation of a Galactokinase from Streptomyces coelicolor Carbohydrate Research 2019, 472, 132-137. https://doi.org/10.1016/j.carres.2018.12.005.
Fallows TW, McGrathAJ, McAdams SG, Marchesi A, Tuna F, Flitsch SL, Tilley RD and Webb SJ. High-throughput Chemical and Chemoenzymatic Approaches to Saccharide-coated Magnetic Nanoparticles for MRI, Nanoscale Advances 2019, 1, 3597-3606DOI: 10.1039/c9na00376. Click here to download.
Huang K, Parmeggiani F,a Ledru H, Hollingsworth K, Mas Pons J, Marchesi A,Both B, Mattey AP, Pallister E, Bulmer GS, van Munster JM, Turnbull WB, Galan MC, Flitsch SL. Enzymatic synthesis of N-acetyllactosamine from Lactose Enabled by Recombinant β1,4- Galactosyltransferases. Organic & Biomolecular Chemistry 2019, 5920-5924. https://doi.org/10.1039/C9OB01089K
cai, ZP, Conway,LP, Huang.YY ,Wang,WJ, Laborda,P, WangT, Lu,AM, Yao,HL, Huang K, Flitsch,SL, Liu,L, & Voglmeir, J, Enzymatic Synthesis of Tri-deuterated Sialosides. Molecules 2019, 24, 1368. https://doi.org/10.3390/molecules24071368
2018
Gray, C and Flitsch, SL. Methods for the High Resolution Analysis of Glycoconjugates. Coupling and Decoupling of Diverse Molecular Units in Glycosciences, 225-267, 2018. ISBN 978-3-319-65587-1
Ahmed, ST, Parmeggiani, F, Weise, NJ, Flitsch, SL and Turner NJ. Engineered Ammonia Lyases for the Production of Challenging Electron Rich L-Phenylalanines. AS Catalysis 8 (4), 312931321 2018. doi: 10.1021/acscatal.8b00496
M Tavanti, JL Porter, S Sabatini, NJ Turner, SL Flitsch. Panel of New Thermostable CYP116B Self‐Sufficient Cytochrome P450 Monooxygenases that Catalyze C− H Activation with a Diverse Substrate Scope. ChemCatChem 10 (5), 10421051, 2018. doi: 10.1002/cctc.201701510
Song, H-B.; He, M.; Cai, Z-P.; Huang, K.; Flitsch, S. L.; Liu, L.; and Voglmeir, J. UDP-Glucose 4-Epimerase and β-1,4-Galactosyltransferase from the Oyster Magallana gigas as Valuable Biocatalysts for the Production of Galactosylated Products. Int. J. Mol. Sci. 2018, 19(6), 1600 doi: 10.3390/ijms19061600
Tavanti, M; Porter, J. L.; Levy, C. W.; Gómez Castellanos, J. R.; Flitsch, S. L.; Turner, N. J. The crystal structure of P450-TT heme-domain provides the first structural insights into the versatile class VII P450s. Biochemical and Biophysical Research Communications, Available online 17 May 2018 doi 10.1016/j.bbrc.2018.05.014
Both, P; Riese, M; Gray, C.J; Huang, K; Pallister, E.G; Kosov, I; Conway, L.P; Voglmeir, J; Flitsch, S. L Applications of a highly α2,6-selective pseudosialidase. Glycobiology 28 (5), 261268, 2018. DOI: doi.org/10.1093/glycob/cwy016
Porter, J.L; Sabatini, S; Manning, J; Tavanti, M; Turner, N.J; Flitsch, S.L. “Characterisation of CYP102A25 from Bacillus marmarensis and CYP102A26 from Pontibacillus halophilus: P450 Homologues of BM3 with Preference towards Hydroxylation of Medium-Chain Fatty Acids. ChemBioChem (2018) DOI 10.1002/cbic.201700598
Porter, J.L; Sabatini, S; Manning, J; Tavanti, M; Galman, J.L; Turner, N.J; Flitsch, S.L. Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope. Enzyme and Microbial Technology (2018) Vol. 113, p1-8. DOI: 10.1016/j.enzmictec.2018.02.005
Craven, F; Silva, J; Segarra-Maset, M; Huang, K; Both, P; Gough, J; Flitsch, S.L.; and Webb, S. ‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes. Chem. Commun., 2018, Accepted manuscript 4 Jan 2018 DOI: 10.1039/C7CC09148F
Huang, K; Parmeggiani, F; Pallister, E; Huang, C-J; Liu, F-F; Li, Q; Birmingham, W.R; Both, P; Thomas, B; Liu, L; Voglmeir, J; and Flitsch, S.L. (2018) Characterisation of a bacterial galactokinase with high activity and broad substrate tolerance for chemoenzymatic synthesis of 6-aminogalactose-1-phosphate and analogues. ChemBioChem. DOI: 10.1002/cbic.201700477
2017
Tavanti, M; Porter, J. L; Sabatini, S; Turner, N. J and Flitsch, S. L. “A panel of new thermostable CYP116B self-sufficient cytochrome P450 monooxygenases catalysing C-H activation with diverse substrate scope”. ChemCatChem, 2017, Accepted manuscript 13th Oct. DOI:10.1002/cctc.201701510
Wood, A. J. L; Weise, N. K; Frampton, J. D; Dunstan, M. S; Hollas, M. A; Derrington, S. R; Lloyd, R. C; Quaglia, D; Parmeggiani, F; Leys, D; Turner, N. J and Flitsch, S. L. “Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides”. Angew. Chemie. Int. Ed, 2017. Accepted Manuscript 21st Sept. DOI: 10.1002/anie.201707918
Both, P; Riese,M; Gray, C. J; Huang, K; Pallister. E. G; Kosov, I; Conway, L. P; Voglmeir, J and Flitsch, S. L. “Development and application of a highly α2,6-selective pseudosialidase.” ChemRxiv, 31.08.2017.
Weissenborn, M, J; Debecker, D. P; Golyen, S; Linclau, B, Turner, N, J and Flitsch, S, L. “Development of a Solid Phase Array Assay for the Screening of Galactose Oxidase Activity and for Fast Identification of Inhibitors.” Protein & Peptide Letters, 2017. Accepted Manuscript 24 July 2017. DOI:10.2174/0929866524666170724114348.
Dunn, B; Huang, Y-Y; Conway, L; He, M; Wei, S; Huang, K; Duan, X-C; Flitsch, S and Volgmeir, J. “Discovery and Biochemical Characterization of a Thermostable Glucose-1-phosphate Nucleotidyltransferase from Thermodesulfatator indicus.” Protein & Peptide Letters, 2017. Accepted Manuscript 24 July 2017. DOI:10.2174/0929866524666170724110408.
Schindler, B; Barnes, L; Renois, G; Gray, C; Allouche, A-R; Chambert, S; Fort, S; Flitsch, S; Loison, C and Compagnon, I. “Anomeric memory of the glycosydic bond upon fragmentation and its consequences for carbohydrate sequencing.” Nature Communications, 2017, 8:973. DOI:10.1038/s41467-017-01179-y
Hollas, Michael A; Webb, Simon; Flitsch, Sabine and Fielding, Alistar J. “A bifunctional spin label for ligand recognition on surfaces.” Angew. Chem. Int. Ed., 2017, 56, 1-6. DOI:10.1002/anie.201703929
Gray, Christopher; Schindler, Baptiste; Migas, Lukasz; Picmanova , Martina ; Allouche, Abdul-Rahman; Green, Anthony; Mandal, Santanu; Motawia, Mohammed; Sánchez-Pérez, Raquel; Bjarnholt , Nanna ; Møller, Birger; Rijs, Anouk; Barran, Perdita; Compagnon, Isabelle; Eyers, Claire; Flitsch, Sabine. “Bottom-up elucidation of glycosidic bond stereochemistry”. Analytical Chemistry, 2017, 89 (8), 4540-4549. DOI: 10.1021/acs.analchem.6b04998
Hepworth, Lorna; France, Scott; Hussain, Shahed; Both, Peter; Turner, Nicholas; Flitsch, Sabine. De Novo Enzyme Cascades in Whole Cells for the Synthesis of Chiral Cyclic Amines.” ACS Catalysis, 2017, 7, 2920-2925. DOI: 10.1021/acscatal.7b00513
Gray, Christopher; Sánchez-Ruiz, Antonio; Šardzíková, Ivana; Ahmed, Yassir; Miller, Rebecca; Reyes Martinez, Juana; Pallister, Edward; Huang, Kun; Both, Peter; Hartmann, Mirja; Roberts, Hannah; Šardzik, Robert; Mandal, Santanu; Turnbull, Jeremy; Eyers, Claire; Flitsch, Sabine. “Label-Free Discovery Array Platform for the Characterization of Glycan Binding Proteins and Glycoproteins” Analytical Chemistry, 2017, 89(8), 4444-4451. DOI: 10.1021/acs.analchem.6b04122
Baptiste Thomas, Xiaojie Lu, William R. Birmingham, Kun Huang, Peter Both, Juana Elizabeth Reyes Martinez, Robert J. Young, Christopher P. Davie and Sabine L. Flitsch. “Application of biocatalysis to on-DNA carbohydrate library synthesis.” ChemBioChem 2017, 18, 1-7. DOI:10.1002/cbic.201600678.
Baptiste Schindler, Loic Barnes, Christopher John Gray, Stephane Chambert, Sabine L. Flitsch, Jos Oomens, Regis Daniel, abdul-Rahman Allouche and Isabelle Compagnon. “IRMPD spectroscopy sheds new (InfraRed) light on the sulfate pattern of carbohydrates.” J. Phys. Chem. A., 2017, 121(10), 2114-2120. DOI:10.1021/acs.jpca.6b11642.
Jolanda M van Munster, Baptiste Thomas, Michel Riese, Adrienne L Davis, Christopher J Gray, David B Archer and Sabine L Flitsch. “Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger.” Scientific Reports, 2017, 43117. DOI:10.1038/srep43117.
Jan M. Klenk, Bernd A. Nebel, Joanne L. Porter, Justyna K. Kulig, Shaneela A. Hussain, Sven M. Richter, Michele Tavanti, Nicolas J. Turner, Martin A. Hayes, Bernhard Hauer, Sabine L. Flitsch. “The self-sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5-hydroxylation of diclofenac.” Biotechnology Journal, 2017, 12(3), 1600520. DOI: 10.1002/biot.201600520.
Yan, Cunyu; Parmeggiani, Fabio; Jones, Emrys A; Claude, Emmanuelle; Hussain, Shaneela A; Turner, Nicholas J; Flitsch, Sabine L; Barran, Perdita E; “Real-time screening of biocatalysts in live bacterial colonies.” J. Am. Chem. Soc., 2017, 139(4), 1408-1411. DOI: 10.1021/jacs.6b12165
France, Scott P; Hepworth, Lorna J; Turner, Nicholas J; Flitsch, Sabine L; “Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways.” ACS Catal., 2017, 7(1), 710-724. DOI: 10.1021/acscatal.6b02979
Ly Y. M, Laborda P, Huang K, Cai P, Wang A. M, Lu C, Doherty C, Liu L, Flitsch S. L and Voglmeir J. “Highly efficient and selective biocatalytic production of glucosamine from chitin.” Green Chemistry, 2017, 19, 527. DOI: 10.1039/c6gc02910h
2016
Ahmed, Syed T.; Parmeggiani, F.; Turner, Nicholas J.; Weise, Nicholas.; Flitsch, Sabine L. “Synthesis of Enantiomerically Pure Ring-Substituted l-Pyridylalanines by Biocatalytic Hydroamination”. Org. Lett, 2016, 18(21), 5468-5471. DOI: 10.1021/acs.orglett.6b02559
France, Scott P.; Hussain, Shahed; Hill, Andrew M.; Hepworth, Lorna J.; Howard, Roger M.; Mulholland, Keith R.; Flitsch, Sabine L.; Turner, Nicholas J. “One-Pot Cascade Synthesis of Mono- and Disubstituted Piperidines and Pyrrolidines using Carboxylic Acid Reductase (CAR), ω-Transaminase (ω-TA), and Imine Reductase (IRED) Biocatalysts”. ACS Catalysis (2016), 6(6), 3753-3759. DOI:10.1021/acscatal.6b00855
Castangia, S. R. Hudson, H. K. Robinson, S. L. Flitsch, J. Thomas-Oates and A. Routledge.“Fabrication and Application of Isotopically-Labelled Gold Arrays for Multiplexed Peptide Analysis.” CHEMBIOCHEM. 2016, 17(21), 3, 2007-2011. DOI:10.1002/cbic.201600347
Eichler A, Gricman Ł, Herter S, Kelly PP, Turner NJ, Pleiss J, Flitsch SL. “Enantioselective Benzylic Hydroxylation Catalysed by P450 Monooxygenases: Characterisation of a P450cam Mutant Library and Molecular Modelling”. CHEMBIOCHEM. 2016 MAR 2;17(5):426-32. DOI: 10.1002/cbic.201500536.
Weissenborn, M. J.; Notonier, S; Lang, S-L; Otte, K. B.; Herter, S; Turner, N. J.; Flitsch, S. L.; Hauer, B. “Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s“. CHEM. COMMUN., 2016, 52(36), 6158-6161. DOI:10.1039/C6CC01749E
Yan, Cunyu; Schmidberger, Jason W.; Parmeggiani, Fabio; Hussain, Shaneela A.; Turner, Nicholas J.; Flitsch, Sabine L.; Barran, Perdita. “Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry“. ANALYST, 2016, 141(8), 2351-2355. DOI:10.1039/C6AN00617E.
SYNFACTS Highlights in Current Synthetic Organic Chemistry. SYNFACTS, 2016, 12(08), 0863. DOI:10.1055/s-0035-156262693.
SYNFACTS Highlights in Current Synthetic Organic Chemistry. SYNFACTS, 2016, 12(3), 0307. DOI:10.1055/s-0035-1561267.
Formisano N, Bhalla N, Heeran M, Martinez J, Sarkar A, Laabei M, Pawan J, Bowen C, Taylor J, Flitsch S and Estrela P. “Inexpensive and fast pathogenic bacteria screening using field-effect transistors”. BIOSENSORS AND BIOELECTRONICS – APR 20 2016, 85, 15, 103-109. DOI:10.1016/j.bios.2016.04.063
Gray C. J; Thomas B, Upton R, Migas L. G, Eyers C. E, Barran P. E and Flitsch S. L. “Applications of ion mobility mass spectrometry for high throughput, high resolution glycan analysis.” BIOCHIMICA AT BIOPHYSICA ACTA (BBA) – GENERAL SUBJECTS. DOI: 10.1016/J.BBAGEN.2016.02.003 – FEB 6 2016.
Both, H. Busch, P. Kelly, F. G. Mutti, N. J. Turner, S. L. Flitsch, Sabine J. “Whole-Cell Biocatalysts for Stereoselective C-H Amination Reactions”. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION., 55; 1511 – 1513.DOI: 10.1002/anie.201510028– JAN 22 2016
2015
Huang, Kun; Wang, Mao M.; Kulinich, Anna; Yao, Hong L.; Ma, Hong Y.; Martinez, Juana E. R.; Duan, Xu C.; Chen, Huan; Cai, Zhi P.; Flitsch, Sabine L.; Liu, Li; Voglmeir, Josef. ”Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila” CARBOHYDRATE RESEARCH. 415; 60-65. DOI 10.1016/j.carres.2015.08.001 – OCT 13 2015
Kelly, Paul P.; Eichler, Anja; Herter, Susanne; Kranz, David C.; Turner, Nicholas J.; Flitsch, Sabine L. “Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 11; 1713-1720. DOI 10.3762/bjoc.11.186 -SEP 22 2015
Ahmed, Syed T.; Parmeggiani, Fabio; Weise, Nicholas J.; Flitsch, Sabine L.; Turner, Nicholas J. “Chemoenzymatic Synthesis of Optically Pure L- and D-Biarylalanines through Biocatalytic Asymmetric Amination and Palladium-Catalyzed Arylation” ACS CATALYSIS. 5(9); 5410 – 5413. DOI 10.1021/acscatal.5b01132 – SEP 2015
Picmanova, Martina; Neilson, Elizabeth H.; Motawia, Mohammed S.; Olsen, Carl Erik; Agerbirk, Niels; Gray, Christopher J.; Flitsch, Sabine; Meier, Sebastian; Silvestro, Daniele; Jorgensen, Kirsten; Sanchez-Perez, Raquel; Moller, Birger Lindberg; Bjarnholt, Nanna. “A recycling pathway for cyanogenic glycosides evidenced by the comparative metabolic profiling in three cyanogenic plant species” BIOCHEMICAL JOURNAL. 469; 375 – 389. DOI 10.1042/BJ20150390 – AUG 1 2015
Bertozzi, Carolyn R.; Stockwell, Brent R.; Kubicek, Stefan; Dickinson, Bryan C.; Chang, Christopher J.; Schultz, Carsten; Silver, Pamela A.; Gestwicki, Jason E.; Chiosis, Gabriela; Chattopadhyay, Amitabha; Butcher, Rebecca A.; Park, Seung Bum; Shoichet, Brian K.; Flitsch, Sabine L.; Zhang, Jin; Liu, David R.; Ohnishi, Yasuo; Weerapana, Eranthie; Williams, Allison H.; He, Chuan; Moroni, Anna; Thiel, Gerhard; Chang, Young-Tae; Waldmann, Herbert; Bogyo, Matthew; Oddershede, Lene B.; Christopoulos, Arthur; Imperiali, Barbara; Ehrlich, Hermann; Chen, Xing; Prescher, Jennifer A. “Voices of chemical biology” NATURE CHEMICAL BIOLOGY. 11(6); 378 – 379. DOI 10.1038/nchembio.1820 – JUN 2015
Voglmeir, Josef; Laurent, Nicolas; Flitsch, Sabine L.; Oelgeschlaeger, Michael; Wilson, Iain B. H. “Biological and biochemical properties of two Xenopus laevis N-acetylgalactosaminyltransferases with contrasting roles in Embryogenesis” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY. 180; 40 – 47. DOI 10.1016/j.cbpb.2014.10.003 – FEB 2015
2014
Noble, Gavin T.; Craven, Faye L.; Segarra-Maset, Maria Dolores; Martinez, Juana Elizabeth Reyes; Sardzik, Robert; Flitsch, Sabine L.; Webb, Simon J. “Sialylation of lactosyl lipids in membrane microdomains by T. cruzi trans-sialidase” ORGANIC & BIOMOLECULAR CHEMISTRY. 12(45); 9272 – 9278. DOI 10.1039/c4ob01852d – 2014
Flitsch, Sabine L. “Development of new synthetic and analytical tools in glycobiotechnology” NEW BIOTECHNOLOGY. 31; S16 – S16. DOI 10.1016/j.nbt.2014.05.1648 – JUL 2014
Cai, Zhi Peng; Hagan, Andrew Kevin; Wang, Mao Mao; Flitsch, Sabine Lahja; Liu, Li; Voglmeir, Josef. “ 2-Pyridylfuran: A New Fluorescent Tag for the Analysis of Carbohydrates” ANALYTICAL CHEMISTRY. 86(10); 5179 – 5186. DOI 10.1021/ac501393a – MAY 20 2014
Both, P.; Green, A. P.; Gray, C. J.; Sardzik, R.; Voglmeir, J.; Fontana, C.; Austeri, M.; Rejzek, M.; Richardson, D.; Field, R. A.; Widmalm, G.; Flitsch, S. L.; Eyers, C. E. “Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing” NATURE CHEMISTRY. 6(1); 65 – 74. DOI 10.1038/NCHEM.1817 – JAN 2014
Olkhov, Rouslan V.; Weissenborn, Martin J.; Flitsch, Sabine L.; Shaw, Andrew M. “Glycosylation Characterization of Human and Porcine Fibrinogen Proteins by Lectin-Binding Biophotonic Microarray Imaging” ANALYTICAL CHEMISTRY. 86(1); 621 – 628. DOI 10.1021/ac402872t – JAN 7 2014
2013
Gray, Christopher J.; Weissenborn, Martin J.; Eyers, Claire E.; Flitsch, Sabine L. “Enzymatic reactions on immobilised substrates” CHEMICAL SOCIETY REVIEWS. 42(15); 6378 – 6405. DOI 10.1039/c3cs60018a – 2013
Serna, Sonia; Hokke, Cornelis H.; Weissenborn, Martin; Flitsch, Sabine; Martin-Lomas, Manuel; Reichardt, Niels-Christian. “Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays” CHEMBIOCHEM. 14(7); 862 – 869. DOI 10.1002/cbic.201300051 – MAY 10 2013
Lopez-Castejon, Gloria; Luheshi, Nadia M.; Compan, Vincent; High, Stephen; Whitehead, Roger C.; Flitsch, Sabine; Kirov, Aleksandr; Prudovsky, Igor; Swanton, Eileithyia; Brough, David. “Deubiquitinases Regulate the Activity of Caspase-1 and Interleukin-1 beta Secretion via Assembly of the Inflammasome” JOURNAL OF BIOLOGICAL CHEMISTRY. 288(4); 2721 – 2733. DOI 10.1074/jbc.M112.422238 – JAN 25 2013
Seeliger, Daniel; Zapater, Cinta; Krenc, Dawid; Haddoub, Rose; Flitsch, Sabine; Beitz, Eric; Cerda, Joan; de Groot, Bert L. “Discovery of Novel Human Aquaporin-1 Blockers” ACS CHEMICAL BIOLOGY. 8(1); 249 – 256. DOI 10.1021/cb300153z – JAN 2013
Martinez, Juana Elizabeth Reyes; Sardzik, Robert; Voglmeir, Josef; Flitsch, Sabine L. “Enzymatic synthesis of colorimetric substrates to determine alpha-2,3-and alpha-2,6-specific neuraminidase activity” RSC ADVANCES. 3(44); 21335 – 21338. DOI 10.1039/c3ra44791j – 2013
O’Reilly, Elaine; Corbett, Mark; Hussain, Shahed; Kelly, Paul P.; Richardson, Dominique; Flitsch, Sabine L.; Turner, Nicholas J. “Substrate promiscuity of cytochrome P450 RhF” CATALYSIS SCIENCE & TECHNOLOGY. 3(6); 1490 – 1492. DOI 10.1039/c3cy00091e – 2013
2012
Castangia, Roberto; Austeri, Martina; Flitsch, Sabine L. “Enzymatic Amine Acyl Exchange in Peptides on Gold Surfaces” ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 51(52); 13016 – 13018. DOI 10.1002/anie.201205404 – 2012
Wehner, Johannes W.; Weissenborn, Martin J.; Hartmann, Mirja; Gray, Christopher J.; Sardzik, Robert; Eyers, Claire E.; Flitsch, Sabine L.; Lindhorst, Thisbe K. “Dual purpose S-trityl-linkers for glycoarray fabrication on both polystyrene and gold” ORGANIC & BIOMOLECULAR CHEMISTRY. 10(44); 8919 – 8926. DOI 10.1039/c2ob26118a – 2012
Noble, Gavin T.; Craven, Faye L.; Voglmeir, Josef; Sardzik, Robert; Flitsch, Sabine L.; Webb, Simon J. “Accelerated Enzymatic Galactosylation of N-Acetylglucosaminolipids in Lipid Microdomains” JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 134(31); 13010 – 13017. DOI 10.1021/ja302506t – AUG 8 2012
Weissenborn, Martin J.; Wehner, Johannes W.; Gray, Christopher J.; Sardzik, Robert; Eyers, Claire E.; Lindhorst, Thisbe K.; Flitsch, Sabine L. “Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 8; 753 – 762. DOI 10.3762/bjoc.8.86 – MAY 21 2012
Weissenborn, Martin J.; Castangia, Roberto; Wehner, Johannes W.; Sardzik, Robert; Lindhorst, Thisbe K.; Flitsch, Sabine L. “Oxo-ester mediated native chemical ligation on microarrays: an efficient and chemoselective coupling methodology” CHEMICAL COMMUNICATIONS. 48(37); 4444 – 4446. DOI 10.1039/c2cc30844d – 2012
O’Reilly, Elaine; Aitken, Suzanne J.; Grogan, Gideon; Kelly, Paul P.; Turner, Nicholas J.; Flitsch, Sabine L. “Regio- and stereoselective oxidation of unactivated C-H bonds with Rhodococcus rhodochrous” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 8; 496 – 500. DOI 10.3762/bjoc.8.56 – APR 3 2012
McKibbin, Craig; Mares, Alina; Piacenti, Michela; Williams, Helen; Roboti, Peristera; Puumalainen, Marjo; Callan, Anna C.; Lesiak-Mieczkowska, Karolina; Linder, Stig; Harant, Hanna; High, Stephen; Flitsch, Sabine L.; Whitehead, Roger C.; Swanton, Eileithyia “Inhibition of protein translocation at the endoplasmic reticulum promotes activation of the unfolded protein response” BIOCHEMICAL JOURNAL. 442; 639 – 648. DOI 10.1042/BJ20111220 – MAR 15 2012
Sardzik, Robert; Green, Anthony P.; Laurent, Nicolas; Both, Peter; Fontana, Carolina; Voglmeir, Josef; Weissenborn, Martin J.; Haddoub, Rose; Grassi, Paola; Haslam, Stuart M.; Widmalm, Goran; Flitsch, Sabine L. “Chemoenzymatic Synthesis of O-Mannosylpeptides in Solution and on Solid Phase” JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 134(10); 4521 – 4524. DOI 10.1021/ja211861m – MAR 14 2012
Laurent, Nicolas; Haddoub, Rose; Voglmeir, Josef; Flitsch, Sabine L.; Chevolot, Y “MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays” CARBOHYDRATE MICROARRAYS: METHODS AND PROTOCOLS (Methods in Molecular Biology). 808; 269 – 284. DOI 10.1007/978-1-61779-373-8_19 – 2012
2011
Robin, Aelig; Koehler, Valentin; Jones, Alison; Ali, Afruja; Kelly, Paul P.; O’Reilly, Elaine; Turner, Nicholas J.; Flitsch, Sabine L. “Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 7; 1494 – 1498. DOI 10.3762/bjoc.7.173 – NOV 2 2011
Ioannou, Avgousta; Cini, Elena; Timofte, Roxana S.; Flitsch, Sabine L.; Turner, Nicholas J.; Linclau, Bruno “Heavily fluorinated carbohydrates as enzyme substrates: oxidation of tetrafluorinated galactose by galactose oxidase” CHEMICAL COMMUNICATIONS. 47(40); 11228 – 11230. DOI 10.1039/c1cc13956h – 2011
Aletrari, Mina-Olga; McKibbin, Craig; Williams, Helen; Pawar, Vidya; Pietroni, Paola; Lord, J. Michael; Flitsch, Sabine L.; Whitehead, Roger; Swanton, Eileithyia; High, Stephen; Spooner, Robert A. “Eeyarestatin 1 Interferes with Both Retrograde and Anterograde Intracellular Trafficking Pathways” PLOS ONE. 6(7); e22713. DOI 10.1371/journal.pone.0022713 – JUL 25 2011
Rannes, Julie B.; Ioannou, Avgousta; Willies, Simon C.; Grogan, Gideon; Behrens, Carsten; Flitsch, Sabine L.; Turner, Nicholas J. “Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution” JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 133(22); 8436 – 8439. DOI 10.1021/ja2018477 – JUN 8 2011
Voglmeir, Josef; Kaloo, Sara; Laurent, Nicolas; Meloni, Marco M.; Bohlmann, Lisa; Wilson, Iain B. H.; Flitsch, Sabine L. “Biochemical correlation of activity of the alpha-dystroglycan-modifying glycosyltransferase POMGnT1 with mutations in muscle-eye-brain disease” BIOCHEMICAL JOURNAL. 436; 447 – 455. DOI 10.1042/BJ20101059 – JUN 1 2011
Sardzik, Robert; Sharma, Ritu; Kaloo, Sara; Voglmeir, Josef; Crocker, Paul R.; Flitsch, Sabine L. “Chemoenzymatic synthesis of sialooligosaccharides on arrays for studies of cell surface adhesion” CHEMICAL COMMUNICATIONS. 47(19); 5425 – 5427. DOI 10.1039/c1cc10745c – 2011
O’Reilly, Elaine; Koehler, Valentin; Flitsch, Sabine L.; Turner, Nicholas J. “Cytochromes P450 as useful biocatalysts: addressing the limitations” CHEMICAL COMMUNICATIONS. 47(9); 2490 – 2501. DOI 10.1039/c0cc03165h – 2011
Sardzik, Robert; Both, Peter; Flitsch, Sabine L. “Post-translational modifications s-linked sugars lost and found” NATURE CHEMICAL BIOLOGY. 7(2); 69 – 70. DOI 10.1038/nchembio.516 – FEB 2011
Alissandratos, Apostolos; Baudendistel, Nina; Hauer, Bernhard; Baldenius, Kai; Flitsch, Sabine; Halling, Peter “Biocompatible functionalisation of starch” CHEMICAL COMMUNICATIONS. 47(2); 683 – 685. DOI 10.1039/c0cc02908d – 2011
2010:
Alissandratos, Apostolos; Baudendistel, Nina; Flitsch, Sabine L.; Hauer, Bernhard; Halling, Peter J. “Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC” BMC BIOTECHNOLOGY. 10; 82. DOI 10.1186/1472-6750-10-82 – NOV 29 2010
Voglmeir, Josef; Sardzik, Robert; Weissenborn, Martin J.; Flitsch, Sabine L. “Enzymatic Glycosylations on Arrays” OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY. 14(4); 437 – 444. DOI 10.1089/omi.2010.0035 – AUG 2010
Sardzik, Robert; Noble, Gavin T.; Weissenborn, Martin J.; Martin, Andrew; Webb, Simon J.; Flitsch, Sabine L. “Preparation of aminoethyl glycosides for glycoconjugation” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 6; 699 – 703. DOI 10.3762/bjoc.6.81 – JUL 29 2010
Sabbadin, Federico; Hyde, Ralph; Robin, Aelig; Hilgarth, Eva-Maria; Delenne, Marie; Flitsch, Sabine; Turner, Nicholas; Grogan, Gideon; Bruce, Neil C. “LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochrome P450s” CHEMBIOCHEM. 11(7); 987 – 994. DOI 10.1002/cbic.201000104 – MAY 3 2010
Tey, Lai-Hock; Loveridge, E. Joel; Swanwick, Richard S.; Flitsch, Sabine L.; Allemann, Rudolf K. “Highly site-selective stability increases by glycosylation of dihydrofolate reductase” FEBS JOURNAL. 277(9); 2171 – 2179. DOI 10.1111/j.1742-4658.2010.07634.x – MAY 2010
2019
Hepworth J, Flitsch S, Remote control with engineered enzymes. Science 10 May 2019, Vol. 364, Issue 6440, pp. 529.
Reprint: htp://science.sciencemag.org/cgi/rapidpdf/364/6440/529?ijkey=XTc2EXXyr/kuA&keytype=ref&siteid=sci
Huang K, Parmeggiani F,a Ledru H, Hollingsworth K, Mas Pons J, Marchesi A,Both B, Mattey Pallister E, Bulmer G,a van Munster J, Turnbull WB, Galan C, Flitsch S “Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4- galactosyltransferases“. Organic & Biomolecular Chemistry, 2019, DOI: 10.1039/C9OB01089K
Cai ZP,, Conway, L, Huang. Y ,Wang, W, Laborda,P, Wang T, Lu, A, Yao,H, Huang, K, Flitsch, S, Liu,L, & Voglmeir, J, “Enzymatic synthesis of tri-deuterated sialosides” Molecules Medicinal Chemistry Symposium 2019: Facing Novel Challenges in Drug Discovery, Accepted manuscript 2 April 2019.
Manning, J, Tavanti, M, Porter, JL, Kress, N, De Visserb, S, Turner N & Flitsch,S,: “Regio- and Enantio-selective Chemo-enzymatic C-H-lactonization of Decanoic Acid to (S)-δ-Decalactone”. Angewandte Chemie, a journal of the Gesellschaft Deutscher Chemiker (German Chemical Society, GDCh), accepted manuscript 5 March 2019.
Helms, V, High, S, Zimmermann, R & Cavalie, A. “Eeyarestatin compounds selectively enhance Sec61-mediated Ca2+ leakage from the endoplasmic reticulum” Cell Chemical Biology, Accepted manuscript 24 January 2019.
2018
Keenan, T; Mills, R; Pocock, E; Budhadev, D; Parmeggiani, F; Flitsch, S;and Fascione, M; “The characterisation of a galactokinase from Streptomyces coelicolor” Carbohydrate Research, 2018, Accepted manuscript 8 December 2018.
Gray, C and Flitsch, SL. “Methods for the High Resolution Analysis of Glycoconjugates.” Coupling and Decoupling of Diverse Molecular Units in Glycosciences, 225-267, 2018. ISBN 978-3-319-65587-1
Ahmed, ST, Parmeggiani, F, Weise, NJ, Flitsch, SL and Turner NJ. “Engineered Ammonia Lyases for the Production of Challenging Electron Rich L-Phenylalanines.” ACS Catalysis 8 (4), 312931321 2018. doi: 10.1021/acscatal.8b00496
M Tavanti, JL Porter, S Sabatini, NJ Turner, SL Flitsch. “Panel of New Thermostable CYP116B Self‐Sufficient Cytochrome P450
Monooxygenases that Catalyze C− H Activation with a Diverse Substrate Scope.” ChemCatChem 10 (5), 10421051, 2018. doi: 10.1002/cctc.201701510
Song, H-B.; He, M.; Cai, Z-P.; Huang, K.; Flitsch, S. L.; Liu, L.; and Voglmeir, J. “UDP-Glucose 4-Epimerase and β-1,4-Galactosyltransferase from the Oyster Magallana gigas as Valuable Biocatalysts for the Production of Galactosylated Products.” Int. J. Mol. Sci. 2018, 19(6), 1600 doi: 10.3390/ijms19061600
Tavanti, M; Porter, J. L.; Levy, C. W.; Gómez Castellanos, J. R.; Flitsch, S. L.; Turner, N. J.; “The crystal structure of P450-TT heme-domain provides the first structural insights into the versatile class VII P450s” Biochemical and Biophysical Research Communications, Available online 17 May 2018 doi 10.1016/j.bbrc.2018.05.014
Both, P; Riese, M; Gray, C.J; Huang, K; Pallister, E.G; Kosov, I; Conway, L.P; Voglmeir, J; Flitsch, S. L. “Applications of a highly α2,6-selective pseudosialidase.” Glycobiology 28 (5), 261268, 2018. DOI: doi.org/10.1093/glycob/cwy016
Porter, J.L; Sabatini, S; Manning, J; Tavanti, M; Turner, N.J; Flitsch, S.L. “Characterisation of CYP102A25 from Bacillus marmarensis and CYP102A26 from Pontibacillus halophilus: P450 Homologues of BM3 with Preference towards Hydroxylation of Medium-Chain Fatty Acids.” ChemBioChem (2018) DOI 10.1002/cbic.201700598
Porter, J.L; Sabatini, S; Manning, J; Tavanti, M; Galman, J.L; Turner, N.J; Flitsch, S.L. “Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope.” Enzyme and Microbial Technology (2018) Vol. 113, p1-8. DOI: 10.1016/j.enzmictec.2018.02.005
Craven, F; Silva, J; Segarra-Maset, M; Huang, K; Both, P; Gough, J; Flitsch, S.L.; and Webb, S.; “‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes” Chem. Commun., 2018, Accepted manuscript 4 Jan 2018 DOI: 10.1039/C7CC09148F
Huang, K; Parmeggiani, F; Pallister, E; Huang, C-J; Liu, F-F; Li, Q; Birmingham, W.R; Both, P; Thomas, B; Liu, L; Voglmeir, J; and Flitsch, S.L. (2018) “Characterisation of a bacterial galactokinase with high activity and broad substrate tolerance for chemoenzymatic synthesis of 6-aminogalactose-1-phosphate and analogues” ChemBioChem DOI: 10.1002/cbic.201700477
2017
Tavanti, M; Porter, J. L; Sabatini, S; Turner, N. J and Flitsch, S. L. “A panel of new thermostable CYP116B self-sufficient cytochrome P450 monooxygenases catalysing C-H activation with diverse substrate scope”. ChemCatChem, 2017, Accepted manuscript 13th Oct. DOI:10.1002/cctc.201701510
Wood, A. J. L; Weise, N. K; Frampton, J. D; Dunstan, M. S; Hollas, M. A; Derrington, S. R; Lloyd, R. C; Quaglia, D; Parmeggiani, F; Leys, D; Turner, N. J and Flitsch, S. L. “Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides”. Angew. Chemie. Int. Ed, 2017. Accepted Manuscript 21st Sept. DOI: 10.1002/anie.201707918
Both, P; Riese,M; Gray, C. J; Huang, K; Pallister. E. G; Kosov, I; Conway, L. P; Voglmeir, J and Flitsch, S. L. “Development and application of a highly α2,6-selective pseudosialidase.” ChemRxiv, 31.08.2017.
Weissenborn, M, J; Debecker, D. P; Golyen, S; Linclau, B, Turner, N, J and Flitsch, S, L. “Development of a Solid Phase Array Assay for the Screening of Galactose Oxidase Activity and for Fast Identification of Inhibitors.” Protein & Peptide Letters, 2017. Accepted Manuscript 24 July 2017. DOI:10.2174/0929866524666170724114348.
Dunn, B; Huang, Y-Y; Conway, L; He, M; Wei, S; Huang, K; Duan, X-C; Flitsch, S and Volgmeir, J. “Discovery and Biochemical Characterization of a Thermostable Glucose-1-phosphate Nucleotidyltransferase from Thermodesulfatator indicus.” Protein & Peptide Letters, 2017. Accepted Manuscript 24 July 2017. DOI:10.2174/0929866524666170724110408.
Schindler, B; Barnes, L; Renois, G; Gray, C; Allouche, A-R; Chambert, S; Fort, S; Flitsch, S; Loison, C and Compagnon, I. “Anomeric memory of the glycosydic bond upon fragmentation and its consequences for carbohydrate sequencing.” Nature Communications, 2017, 8:973. DOI:10.1038/s41467-017-01179-y
Hollas, Michael A; Webb, Simon; Flitsch, Sabine and Fielding, Alistar J. “A bifunctional spin label for ligand recognition on surfaces.” Angew. Chem. Int. Ed., 2017, 56, 1-6. DOI:10.1002/anie.201703929
Gray, Christopher; Schindler, Baptiste; Migas, Lukasz; Picmanova , Martina ; Allouche, Abdul-Rahman; Green, Anthony; Mandal, Santanu; Motawia, Mohammed; Sánchez-Pérez, Raquel; Bjarnholt , Nanna ; Møller, Birger; Rijs, Anouk; Barran, Perdita; Compagnon, Isabelle; Eyers, Claire; Flitsch, Sabine. “Bottom-up elucidation of glycosidic bond stereochemistry”. Analytical Chemistry, 2017, 89 (8), 4540-4549. DOI: 10.1021/acs.analchem.6b04998
Hepworth, Lorna; France, Scott; Hussain, Shahed; Both, Peter; Turner, Nicholas; Flitsch, Sabine. De Novo Enzyme Cascades in Whole Cells for the Synthesis of Chiral Cyclic Amines.” ACS Catalysis, 2017, 7, 2920-2925. DOI: 10.1021/acscatal.7b00513
Gray, Christopher; Sánchez-Ruiz, Antonio; Šardzíková, Ivana; Ahmed, Yassir; Miller, Rebecca; Reyes Martinez, Juana; Pallister, Edward; Huang, Kun; Both, Peter; Hartmann, Mirja; Roberts, Hannah; Šardzik, Robert; Mandal, Santanu; Turnbull, Jeremy; Eyers, Claire; Flitsch, Sabine. “Label-Free Discovery Array Platform for the Characterization of Glycan Binding Proteins and Glycoproteins” Analytical Chemistry, 2017, 89(8), 4444-4451. DOI: 10.1021/acs.analchem.6b04122
Baptiste Thomas, Xiaojie Lu, William R. Birmingham, Kun Huang, Peter Both, Juana Elizabeth Reyes Martinez, Robert J. Young, Christopher P. Davie and Sabine L. Flitsch. “Application of biocatalysis to on-DNA carbohydrate library synthesis.” ChemBioChem 2017, 18, 1-7. DOI:10.1002/cbic.201600678.
Baptiste Schindler, Loic Barnes, Christopher John Gray, Stephane Chambert, Sabine L. Flitsch, Jos Oomens, Regis Daniel, abdul-Rahman Allouche and Isabelle Compagnon. “IRMPD spectroscopy sheds new (InfraRed) light on the sulfate pattern of carbohydrates.” J. Phys. Chem. A., 2017, 121(10), 2114-2120. DOI:10.1021/acs.jpca.6b11642.
Jolanda M van Munster, Baptiste Thomas, Michel Riese, Adrienne L Davis, Christopher J Gray, David B Archer and Sabine L Flitsch. “Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger.” Scientific Reports, 2017, 43117. DOI:10.1038/srep43117.
Jan M. Klenk, Bernd A. Nebel, Joanne L. Porter, Justyna K. Kulig, Shaneela A. Hussain, Sven M. Richter, Michele Tavanti, Nicolas J. Turner, Martin A. Hayes, Bernhard Hauer, Sabine L. Flitsch. “The self-sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5-hydroxylation of diclofenac.” Biotechnology Journal, 2017, 12(3), 1600520. DOI: 10.1002/biot.201600520.
Yan, Cunyu; Parmeggiani, Fabio; Jones, Emrys A; Claude, Emmanuelle; Hussain, Shaneela A; Turner, Nicholas J; Flitsch, Sabine L; Barran, Perdita E; “Real-time screening of biocatalysts in live bacterial colonies.” J. Am. Chem. Soc., 2017, 139(4), 1408-1411. DOI: 10.1021/jacs.6b12165
France, Scott P; Hepworth, Lorna J; Turner, Nicholas J; Flitsch, Sabine L; “Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways.” ACS Catal., 2017, 7(1), 710-724. DOI: 10.1021/acscatal.6b02979
Ly Y. M, Laborda P, Huang K, Cai P, Wang A. M, Lu C, Doherty C, Liu L, Flitsch S. L and Voglmeir J. “Highly efficient and selective biocatalytic production of glucosamine from chitin.” Green Chemistry, 2017, 19, 527. DOI: 10.1039/c6gc02910h
2016
Ahmed, Syed T.; Parmeggiani, F.; Turner, Nicholas J.; Weise, Nicholas.; Flitsch, Sabine L. “Synthesis of Enantiomerically Pure Ring-Substituted l-Pyridylalanines by Biocatalytic Hydroamination”. Org. Lett, 2016, 18(21), 5468-5471. DOI: 10.1021/acs.orglett.6b02559
France, Scott P.; Hussain, Shahed; Hill, Andrew M.; Hepworth, Lorna J.; Howard, Roger M.; Mulholland, Keith R.; Flitsch, Sabine L.; Turner, Nicholas J. “One-Pot Cascade Synthesis of Mono- and Disubstituted Piperidines and Pyrrolidines using Carboxylic Acid Reductase (CAR), ω-Transaminase (ω-TA), and Imine Reductase (IRED) Biocatalysts”. ACS Catalysis (2016), 6(6), 3753-3759. DOI:10.1021/acscatal.6b00855
Castangia, S. R. Hudson, H. K. Robinson, S. L. Flitsch, J. Thomas-Oates and A. Routledge.“Fabrication and Application of Isotopically-Labelled Gold Arrays for Multiplexed Peptide Analysis.” CHEMBIOCHEM. 2016, 17(21), 3, 2007-2011. DOI:10.1002/cbic.201600347
Eichler A, Gricman Ł, Herter S, Kelly PP, Turner NJ, Pleiss J, Flitsch SL. “Enantioselective Benzylic Hydroxylation Catalysed by P450 Monooxygenases: Characterisation of a P450cam Mutant Library and Molecular Modelling”. CHEMBIOCHEM. 2016 MAR 2;17(5):426-32. DOI: 10.1002/cbic.201500536.
Weissenborn, M. J.; Notonier, S; Lang, S-L; Otte, K. B.; Herter, S; Turner, N. J.; Flitsch, S. L.; Hauer, B. “Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s“. CHEM. COMMUN., 2016, 52(36), 6158-6161. DOI:10.1039/C6CC01749E
Yan, Cunyu; Schmidberger, Jason W.; Parmeggiani, Fabio; Hussain, Shaneela A.; Turner, Nicholas J.; Flitsch, Sabine L.; Barran, Perdita. “Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry“. ANALYST, 2016, 141(8), 2351-2355. DOI:10.1039/C6AN00617E.
SYNFACTS Highlights in Current Synthetic Organic Chemistry. SYNFACTS, 2016, 12(08), 0863. DOI:10.1055/s-0035-156262693.
SYNFACTS Highlights in Current Synthetic Organic Chemistry. SYNFACTS, 2016, 12(3), 0307. DOI:10.1055/s-0035-1561267.
Formisano N, Bhalla N, Heeran M, Martinez J, Sarkar A, Laabei M, Pawan J, Bowen C, Taylor J, Flitsch S and Estrela P. “Inexpensive and fast pathogenic bacteria screening using field-effect transistors”. BIOSENSORS AND BIOELECTRONICS – APR 20 2016, 85, 15, 103-109. DOI:10.1016/j.bios.2016.04.063
Gray C. J; Thomas B, Upton R, Migas L. G, Eyers C. E, Barran P. E and Flitsch S. L. “Applications of ion mobility mass spectrometry for high throughput, high resolution glycan analysis.” BIOCHIMICA AT BIOPHYSICA ACTA (BBA) – GENERAL SUBJECTS. DOI: 10.1016/J.BBAGEN.2016.02.003 – FEB 6 2016.
Both, H. Busch, P. Kelly, F. G. Mutti, N. J. Turner, S. L. Flitsch, Sabine J. “Whole-Cell Biocatalysts for Stereoselective C-H Amination Reactions”. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION., 55; 1511 – 1513.DOI: 10.1002/anie.201510028– JAN 22 2016
2015
Huang, Kun; Wang, Mao M.; Kulinich, Anna; Yao, Hong L.; Ma, Hong Y.; Martinez, Juana E. R.; Duan, Xu C.; Chen, Huan; Cai, Zhi P.; Flitsch, Sabine L.; Liu, Li; Voglmeir, Josef. ”Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila” CARBOHYDRATE RESEARCH. 415; 60-65. DOI 10.1016/j.carres.2015.08.001 – OCT 13 2015
Kelly, Paul P.; Eichler, Anja; Herter, Susanne; Kranz, David C.; Turner, Nicholas J.; Flitsch, Sabine L. “Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 11; 1713-1720. DOI 10.3762/bjoc.11.186 -SEP 22 2015
Ahmed, Syed T.; Parmeggiani, Fabio; Weise, Nicholas J.; Flitsch, Sabine L.; Turner, Nicholas J. “Chemoenzymatic Synthesis of Optically Pure L- and D-Biarylalanines through Biocatalytic Asymmetric Amination and Palladium-Catalyzed Arylation” ACS CATALYSIS. 5(9); 5410 – 5413. DOI 10.1021/acscatal.5b01132 – SEP 2015
Picmanova, Martina; Neilson, Elizabeth H.; Motawia, Mohammed S.; Olsen, Carl Erik; Agerbirk, Niels; Gray, Christopher J.; Flitsch, Sabine; Meier, Sebastian; Silvestro, Daniele; Jorgensen, Kirsten; Sanchez-Perez, Raquel; Moller, Birger Lindberg; Bjarnholt, Nanna. “A recycling pathway for cyanogenic glycosides evidenced by the comparative metabolic profiling in three cyanogenic plant species” BIOCHEMICAL JOURNAL. 469; 375 – 389. DOI 10.1042/BJ20150390 – AUG 1 2015
Bertozzi, Carolyn R.; Stockwell, Brent R.; Kubicek, Stefan; Dickinson, Bryan C.; Chang, Christopher J.; Schultz, Carsten; Silver, Pamela A.; Gestwicki, Jason E.; Chiosis, Gabriela; Chattopadhyay, Amitabha; Butcher, Rebecca A.; Park, Seung Bum; Shoichet, Brian K.; Flitsch, Sabine L.; Zhang, Jin; Liu, David R.; Ohnishi, Yasuo; Weerapana, Eranthie; Williams, Allison H.; He, Chuan; Moroni, Anna; Thiel, Gerhard; Chang, Young-Tae; Waldmann, Herbert; Bogyo, Matthew; Oddershede, Lene B.; Christopoulos, Arthur; Imperiali, Barbara; Ehrlich, Hermann; Chen, Xing; Prescher, Jennifer A. “Voices of chemical biology” NATURE CHEMICAL BIOLOGY. 11(6); 378 – 379. DOI 10.1038/nchembio.1820 – JUN 2015
Voglmeir, Josef; Laurent, Nicolas; Flitsch, Sabine L.; Oelgeschlaeger, Michael; Wilson, Iain B. H. “Biological and biochemical properties of two Xenopus laevis N-acetylgalactosaminyltransferases with contrasting roles in Embryogenesis” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY. 180; 40 – 47. DOI 10.1016/j.cbpb.2014.10.003 – FEB 2015
2014
Noble, Gavin T.; Craven, Faye L.; Segarra-Maset, Maria Dolores; Martinez, Juana Elizabeth Reyes; Sardzik, Robert; Flitsch, Sabine L.; Webb, Simon J. “Sialylation of lactosyl lipids in membrane microdomains by T. cruzi trans-sialidase” ORGANIC & BIOMOLECULAR CHEMISTRY. 12(45); 9272 – 9278. DOI 10.1039/c4ob01852d – 2014
Flitsch, Sabine L. “Development of new synthetic and analytical tools in glycobiotechnology” NEW BIOTECHNOLOGY. 31; S16 – S16. DOI 10.1016/j.nbt.2014.05.1648 – JUL 2014
Cai, Zhi Peng; Hagan, Andrew Kevin; Wang, Mao Mao; Flitsch, Sabine Lahja; Liu, Li; Voglmeir, Josef. “ 2-Pyridylfuran: A New Fluorescent Tag for the Analysis of Carbohydrates” ANALYTICAL CHEMISTRY. 86(10); 5179 – 5186. DOI 10.1021/ac501393a – MAY 20 2014
Both, P.; Green, A. P.; Gray, C. J.; Sardzik, R.; Voglmeir, J.; Fontana, C.; Austeri, M.; Rejzek, M.; Richardson, D.; Field, R. A.; Widmalm, G.; Flitsch, S. L.; Eyers, C. E. “Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing” NATURE CHEMISTRY. 6(1); 65 – 74. DOI 10.1038/NCHEM.1817 – JAN 2014
Olkhov, Rouslan V.; Weissenborn, Martin J.; Flitsch, Sabine L.; Shaw, Andrew M. “Glycosylation Characterization of Human and Porcine Fibrinogen Proteins by Lectin-Binding Biophotonic Microarray Imaging” ANALYTICAL CHEMISTRY. 86(1); 621 – 628. DOI 10.1021/ac402872t – JAN 7 2014
2013
Gray, Christopher J.; Weissenborn, Martin J.; Eyers, Claire E.; Flitsch, Sabine L. “Enzymatic reactions on immobilised substrates” CHEMICAL SOCIETY REVIEWS. 42(15); 6378 – 6405. DOI 10.1039/c3cs60018a – 2013
Serna, Sonia; Hokke, Cornelis H.; Weissenborn, Martin; Flitsch, Sabine; Martin-Lomas, Manuel; Reichardt, Niels-Christian. “Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays” CHEMBIOCHEM. 14(7); 862 – 869. DOI 10.1002/cbic.201300051 – MAY 10 2013
Lopez-Castejon, Gloria; Luheshi, Nadia M.; Compan, Vincent; High, Stephen; Whitehead, Roger C.; Flitsch, Sabine; Kirov, Aleksandr; Prudovsky, Igor; Swanton, Eileithyia; Brough, David. “Deubiquitinases Regulate the Activity of Caspase-1 and Interleukin-1 beta Secretion via Assembly of the Inflammasome” JOURNAL OF BIOLOGICAL CHEMISTRY. 288(4); 2721 – 2733. DOI 10.1074/jbc.M112.422238 – JAN 25 2013
Seeliger, Daniel; Zapater, Cinta; Krenc, Dawid; Haddoub, Rose; Flitsch, Sabine; Beitz, Eric; Cerda, Joan; de Groot, Bert L. “Discovery of Novel Human Aquaporin-1 Blockers” ACS CHEMICAL BIOLOGY. 8(1); 249 – 256. DOI 10.1021/cb300153z – JAN 2013
Martinez, Juana Elizabeth Reyes; Sardzik, Robert; Voglmeir, Josef; Flitsch, Sabine L. “Enzymatic synthesis of colorimetric substrates to determine alpha-2,3-and alpha-2,6-specific neuraminidase activity” RSC ADVANCES. 3(44); 21335 – 21338. DOI 10.1039/c3ra44791j – 2013
O’Reilly, Elaine; Corbett, Mark; Hussain, Shahed; Kelly, Paul P.; Richardson, Dominique; Flitsch, Sabine L.; Turner, Nicholas J. “Substrate promiscuity of cytochrome P450 RhF” CATALYSIS SCIENCE & TECHNOLOGY. 3(6); 1490 – 1492. DOI 10.1039/c3cy00091e – 2013
2012
Castangia, Roberto; Austeri, Martina; Flitsch, Sabine L. “Enzymatic Amine Acyl Exchange in Peptides on Gold Surfaces” ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 51(52); 13016 – 13018. DOI 10.1002/anie.201205404 – 2012
Wehner, Johannes W.; Weissenborn, Martin J.; Hartmann, Mirja; Gray, Christopher J.; Sardzik, Robert; Eyers, Claire E.; Flitsch, Sabine L.; Lindhorst, Thisbe K. “Dual purpose S-trityl-linkers for glycoarray fabrication on both polystyrene and gold” ORGANIC & BIOMOLECULAR CHEMISTRY. 10(44); 8919 – 8926. DOI 10.1039/c2ob26118a – 2012
Noble, Gavin T.; Craven, Faye L.; Voglmeir, Josef; Sardzik, Robert; Flitsch, Sabine L.; Webb, Simon J. “Accelerated Enzymatic Galactosylation of N-Acetylglucosaminolipids in Lipid Microdomains” JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 134(31); 13010 – 13017. DOI 10.1021/ja302506t – AUG 8 2012
Weissenborn, Martin J.; Wehner, Johannes W.; Gray, Christopher J.; Sardzik, Robert; Eyers, Claire E.; Lindhorst, Thisbe K.; Flitsch, Sabine L. “Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 8; 753 – 762. DOI 10.3762/bjoc.8.86 – MAY 21 2012
Weissenborn, Martin J.; Castangia, Roberto; Wehner, Johannes W.; Sardzik, Robert; Lindhorst, Thisbe K.; Flitsch, Sabine L. “Oxo-ester mediated native chemical ligation on microarrays: an efficient and chemoselective coupling methodology” CHEMICAL COMMUNICATIONS. 48(37); 4444 – 4446. DOI 10.1039/c2cc30844d – 2012
O’Reilly, Elaine; Aitken, Suzanne J.; Grogan, Gideon; Kelly, Paul P.; Turner, Nicholas J.; Flitsch, Sabine L. “Regio- and stereoselective oxidation of unactivated C-H bonds with Rhodococcus rhodochrous” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 8; 496 – 500. DOI 10.3762/bjoc.8.56 – APR 3 2012
McKibbin, Craig; Mares, Alina; Piacenti, Michela; Williams, Helen; Roboti, Peristera; Puumalainen, Marjo; Callan, Anna C.; Lesiak-Mieczkowska, Karolina; Linder, Stig; Harant, Hanna; High, Stephen; Flitsch, Sabine L.; Whitehead, Roger C.; Swanton, Eileithyia “Inhibition of protein translocation at the endoplasmic reticulum promotes activation of the unfolded protein response” BIOCHEMICAL JOURNAL. 442; 639 – 648. DOI 10.1042/BJ20111220 – MAR 15 2012
Sardzik, Robert; Green, Anthony P.; Laurent, Nicolas; Both, Peter; Fontana, Carolina; Voglmeir, Josef; Weissenborn, Martin J.; Haddoub, Rose; Grassi, Paola; Haslam, Stuart M.; Widmalm, Goran; Flitsch, Sabine L. “Chemoenzymatic Synthesis of O-Mannosylpeptides in Solution and on Solid Phase” JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 134(10); 4521 – 4524. DOI 10.1021/ja211861m – MAR 14 2012
Laurent, Nicolas; Haddoub, Rose; Voglmeir, Josef; Flitsch, Sabine L.; Chevolot, Y “MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays” CARBOHYDRATE MICROARRAYS: METHODS AND PROTOCOLS (Methods in Molecular Biology). 808; 269 – 284. DOI 10.1007/978-1-61779-373-8_19 – 2012
2011
Robin, Aelig; Koehler, Valentin; Jones, Alison; Ali, Afruja; Kelly, Paul P.; O’Reilly, Elaine; Turner, Nicholas J.; Flitsch, Sabine L. “Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 7; 1494 – 1498. DOI 10.3762/bjoc.7.173 – NOV 2 2011
Ioannou, Avgousta; Cini, Elena; Timofte, Roxana S.; Flitsch, Sabine L.; Turner, Nicholas J.; Linclau, Bruno “Heavily fluorinated carbohydrates as enzyme substrates: oxidation of tetrafluorinated galactose by galactose oxidase” CHEMICAL COMMUNICATIONS. 47(40); 11228 – 11230. DOI 10.1039/c1cc13956h – 2011
Aletrari, Mina-Olga; McKibbin, Craig; Williams, Helen; Pawar, Vidya; Pietroni, Paola; Lord, J. Michael; Flitsch, Sabine L.; Whitehead, Roger; Swanton, Eileithyia; High, Stephen; Spooner, Robert A. “Eeyarestatin 1 Interferes with Both Retrograde and Anterograde Intracellular Trafficking Pathways” PLOS ONE. 6(7); e22713. DOI 10.1371/journal.pone.0022713 – JUL 25 2011
Rannes, Julie B.; Ioannou, Avgousta; Willies, Simon C.; Grogan, Gideon; Behrens, Carsten; Flitsch, Sabine L.; Turner, Nicholas J. “Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution” JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 133(22); 8436 – 8439. DOI 10.1021/ja2018477 – JUN 8 2011
Voglmeir, Josef; Kaloo, Sara; Laurent, Nicolas; Meloni, Marco M.; Bohlmann, Lisa; Wilson, Iain B. H.; Flitsch, Sabine L. “Biochemical correlation of activity of the alpha-dystroglycan-modifying glycosyltransferase POMGnT1 with mutations in muscle-eye-brain disease” BIOCHEMICAL JOURNAL. 436; 447 – 455. DOI 10.1042/BJ20101059 – JUN 1 2011
Sardzik, Robert; Sharma, Ritu; Kaloo, Sara; Voglmeir, Josef; Crocker, Paul R.; Flitsch, Sabine L. “Chemoenzymatic synthesis of sialooligosaccharides on arrays for studies of cell surface adhesion” CHEMICAL COMMUNICATIONS. 47(19); 5425 – 5427. DOI 10.1039/c1cc10745c – 2011
O’Reilly, Elaine; Koehler, Valentin; Flitsch, Sabine L.; Turner, Nicholas J. “Cytochromes P450 as useful biocatalysts: addressing the limitations” CHEMICAL COMMUNICATIONS. 47(9); 2490 – 2501. DOI 10.1039/c0cc03165h – 2011
Sardzik, Robert; Both, Peter; Flitsch, Sabine L. “Post-translational modifications s-linked sugars lost and found” NATURE CHEMICAL BIOLOGY. 7(2); 69 – 70. DOI 10.1038/nchembio.516 – FEB 2011
Alissandratos, Apostolos; Baudendistel, Nina; Hauer, Bernhard; Baldenius, Kai; Flitsch, Sabine; Halling, Peter “Biocompatible functionalisation of starch” CHEMICAL COMMUNICATIONS. 47(2); 683 – 685. DOI 10.1039/c0cc02908d – 2011
2010:
Alissandratos, Apostolos; Baudendistel, Nina; Flitsch, Sabine L.; Hauer, Bernhard; Halling, Peter J. “Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC” BMC BIOTECHNOLOGY. 10; 82. DOI 10.1186/1472-6750-10-82 – NOV 29 2010
Voglmeir, Josef; Sardzik, Robert; Weissenborn, Martin J.; Flitsch, Sabine L. “Enzymatic Glycosylations on Arrays” OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY. 14(4); 437 – 444. DOI 10.1089/omi.2010.0035 – AUG 2010
Sardzik, Robert; Noble, Gavin T.; Weissenborn, Martin J.; Martin, Andrew; Webb, Simon J.; Flitsch, Sabine L. “Preparation of aminoethyl glycosides for glycoconjugation” BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY. 6; 699 – 703. DOI 10.3762/bjoc.6.81 – JUL 29 2010
Sabbadin, Federico; Hyde, Ralph; Robin, Aelig; Hilgarth, Eva-Maria; Delenne, Marie; Flitsch, Sabine; Turner, Nicholas; Grogan, Gideon; Bruce, Neil C. “LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochrome P450s” CHEMBIOCHEM. 11(7); 987 – 994. DOI 10.1002/cbic.201000104 – MAY 3 2010
Tey, Lai-Hock; Loveridge, E. Joel; Swanwick, Richard S.; Flitsch, Sabine L.; Allemann, Rudolf K. “Highly site-selective stability increases by glycosylation of dihydrofolate reductase” FEBS JOURNAL. 277(9); 2171 – 2179. DOI 10.1111/j.1742-4658.2010.07634.x – MAY 2010