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- Bell, E.L.; Finnigan, W.; France, S.P.; Green, A.P.; Hayes, M.A.; Hepworth, L.J.; Lovelock, S.L.; Niikura, H.; Osuna, S.; Romero, E.; Ryan, K.S.; Turner, N.J.; Flitsch, S.L. Biocatalysis Nature Reviews Methods Primers 2021, 1, 46
- Mattey, A.P.; Ford, G.J.; Citoler, J.; Baldwin, C.; Marshall, J.R.; Palmer, R.B.; Thompson, M.; Turner, N.J.; Cosgrove, S.C.; Flitsch, S.L. Development of Continuous Flow Systems to Access Secondary Amines Through Previously Incompatible Biocatalytic Cascades Angew. Chem. Int. Ed. 2021, 60(34), 18660
- Kempa, E.E.; Galman, J.L.; Parmeggiani, F.; Marshall, J.R.; Malassis, J.; Fontenelle, C.Q.; Vendeville, J-B.; Linclau, B.; Charnock, S.J.; Flitsch, S.L.; Turner, N.J.; Barran, P.E. Rapid Screening of Diverse Biotransformations for Enzyme Evolution JACS Au 2021, 1(4), 508
- Finnigan, W.; Hepworth, L.J.; Flitsch, S.L.; Turner, N.J. RetroBioCat as a Computer-aided Synthesis Planning Tool for Biocatalytic Reactions and Cascades Nature Catalysis 2021, 4, 98
- Romero, E.; Jones, B.S.; Hogg, B.N.; Casamajo, A.R.; Hayes, M.A.; Flitsch, S.L.; Turner, N.J.; Schnepel, C. Enzymatic Late-Stage Modifications: Better Late Than Never Angew. Chem. Int. Ed. 2021, 60(31), 16824
- Maciej-Hulme, M.L.; Dubaissi, E.; Shao, C.; Zaia, J.; Amaya, E.; Flitsch, S.L.; Merry, C.L.R. Selective Inhibition of Heparan Sulphate and Not Chondroitin Sulphate Biosynthesis by a Small, Soluble Competitive Inhibitor Int. J. Mol. Sci. 2021, 22(13) 6988
- Bulmer, G.S.; Mattey, A.P.; Parmeggiani, F.; Williams, R.; Ledru, H.; Marchesi, A.; Seibt, L.S.; Both, P.; Hunag, K.; Galan, M.C.; Flitsch, S.L.; Green, A.P.; van Munster, J.M. A Promiscuous Glycosyltransferase Generates Poly-beta1,4-glucan Derivatives That Facilitate Mass Spectrometry-based Detection of Cellulolytic Enzymes Org. Biomol. Chem. 2021, 19, 5529
- Finnigan, W.; Flitsch, S.L.; Hepworth, L.J.; Turner, N.J. Enzyme Cascade Design: Retrosynthesis Enzyme Cascade Design and Modelling (S.Kara and F. Rudroff eds) 2021, 7
- Pallister, E.; Choo, M.S.F.; Walsh, I.; Tai, J.N.; Yang, Y.S.; Ng, S.K.; Rudd, P.M.; Flitsch, S.L.; Nguyen-Khuong, T. Utility of Ion-Mobility Spectrometry for Deducing Branching of Multiply Charged Glycans and Glycopeptides in a High-throughput Positive Ion LC-FLR-IMS-MS Workflow Anal. Chem. 2020, 92(23), 15323
- Marchesi, A.; Parmeggiani, F.; Louçano, J.; Mattey, A.P.; Huang, K.; Gupta, T.; Salwiczek, M.; Flitsch, S.L. Enzymatic Building Block Synthesis for Automated Solid Phase Glycan Assembly Angew. Chem. Int. Ed. 2020, 59(50), 22456
- Pallister, E.; Gray, C.J.; Flitsch, S.L. Enzyme Promiscuity of Carbohydrate Active Enzymes and Their Applications in Biocatalysis Current Opinions in Structural Biology 2020, 65, 184
- Lubberink, M.; Schnepel, C.; Citoler, J.; Derrington, S.R.; Finnigan, W.; Hayes, M.A.; Turner, N.J.; Flitsch, S.L. Biocatalytic Monoacylation of Symmetrical Diamines and its Application to the Synthesis of Pharmaceutically Relevant Amides ACS Catalysis 2020, 10(17), 10005
- Weise, N.J.; Thapa, P.; Ahmed, S.T.; Heath, R.S.; Parmeggiani, F.; Turner, N. J.; Flitsch, S.L. Bi-enzymatic Conversion of Cinnamic Acids to 2-Arylethylamines ChemCatChem 2020, 12(4) 995
- Flack, E.K.P.; Chidwick, H.S; Guchhait, G.; Keenan, T.; Budhadev, D.; Huang, K.; Both, P.; Mas Pons, J.; Ledru, H.; Rui, S.; Stafford, G.P.; Shaw, J.G.; Galan, M.C.; Flitsch S.; Thomas, G.H.; Fascione, M.A. Biocatalytic Transfer of Pseudaminic Acid (Pse5Ac7Ac) using Promiscuous Sialyltransferases in a Chemoenzymatic Approach to Pse5Ac7Ac Containing Glycosides ACS Catalysis 2020, 10(17), 9986
- Keenan, T.; Parmeggiani, F.; Malassis, J.; Fontenelle, C.Q.; Vendeville, J.; Offen, W.; Both, P.; Huang, K.; Marchesi, A.; Heyam, A.; Young, C.; Charnock, S.J.; Davies, G.J.; Linclau, B.; Flitsch, S.L.; Fascione, M.A. Profiling Substrate Promiscuity of Wild-type Sugar Kinases for Multi-fluorinated Monosaccharides Cell Chemical Biology 2020, 27, 1
- Gray, C.J.; Compagnon, I.; Flitsch, S.L. Mass Spectrometry Hybridized with Gas-phase InfraRed Spectroscopy for Glycan Sequencing Current Opinion in Structural Biology 2020, 62, 121.
- Shellard, P.M.; Srisubin, T.; Hartmann, M.; Butcher, J.; Fei, F.; Cox, H.; McNamara, T.P.; McArdle, T.; Shepherd, A.M.; Jacobs, R.M.J.; Waigh, T.A.; Flitsch, S.L.; Blanford, C.F. A Versatile Route to Edge-specific Modifications to Pristine Graphene by Electrophilic Aromatic Substitution Journal of Materials Science 2020, 55(24), 10284
- Paggiola, G.; Derrien, N.; Moseley, J. D.; Green, A.; Flitsch, S.L.; Clark, J.H.; McElroy, C.R.; Hunt, A.J. Application of Bio-based Solvents for Biocatalysed Synthesis of Amides with Pseudomonas stutzeri lipase (PSL) Pure and Applied Chemistry 2020, 92(4), 579.
- Louçano, J.; Both, P.; Marchesi, A.; del Bino, L.; Adamo, R.; Flitsch, S.; Salwiczek, M. Automated Glycan Assembly of Streptococcus pneumoniae type 14 Capsular Polysaccharide Fragments RSC Advances 2020, 10(40), 23668
- Ford, G.; Kress, N.; Mattey, A.P.; Hepworth, L.; Baldwin, C.; Marshall, J.; Seibt, L.; Huang, M.; Birmingham, W.; Turner, N.J.; Flitsch, S.L. Synthesis of Protected 3 Aminopiperidine and 3-Aminoazepane Derivatives Using Enzyme Cascades Chem. Commun. 2020, 56, 7949-7952
21. Valverde, P.; Vendeville, J.; Hollingsworth, K.; Mattey, A.P.; Keenan, T.; Chidwick, H.; Ledru, H.; Huonnic, K.; Huang, K.; Light, M.E.; Turner, N.; Jimenez-Barbero, J.; Galan, M.C.; Fascione, M.A.; Flitsch, S.; Turnbull, W.B.; Linclau, B. Chemoenzymatic Synthesis of 3-Deoxy-3-fluoro-L-fucose and its Enzymatic Incorporation into Glycoconjugates Chem. Commun. 2020, 56, 6408
22. Mattey, A.P.; Sangster, J. J.; Ramsden, J. I.; Baldwin, C.; Birmingham, W.R.; Heath, R.S.; Angelastro, A.; Turner, N.J.; Cosgrove, S.C.; Flitsch, S.L. Natural Heterogeneous Catalysis with Immobilised Oxidase Biocatalysts RSC Advances 2020, 10(33), 19501
23. Huang, K.; Marchesi, A.; Hollingsworth, K.; Both, P.; Mattey, A.P.; Pallister, E.; Ledru, H.; Charnock, S.J.; Galan, M.C.; Turnbull,W.B.; Parmeggiani, F.; Flitsch, S.L. Biochemical Characterisation of an 1, 4 Galactosyltransferase from Neisseria Weaveri for the Synthesis of 1, 4-linked Galactosides Organic & Biomolecular Chemistry 2020, 18(16), 3142
24. Cosgrove, S.C.; Mattey, A.P.; Riese, M.; Chapman, M.R.; Birmingham, W. R.; Blacker, A. J.; Kapur, N.; Turner, N.J.; Flitsch, S.L. Biocatalytic Oxidation in Continuous Flow for the Generation of Carbohydrate Dialdehydes ACS Catalysis 2020, 9(12), 11658
25. Laborda, P.; Lyu, Y-M.; Parmeggiani, F.; Lu, A-M.; Wang, W-J.; Huang, Y-Y.; Huang, K.; Guo, J.; Liu, L.; Flitsch, S.L.; Voglmeir, J. An Enzymatic N-Acylation Step Enables the Biocatalytic Synthesis of Unnatural Sialosides Angew. Chem. Int. Ed. 2020, 132(13), 5346
26. Gray, C.J.; Migas, L.G.; Barran, P.E.; Pagel, K.; Seeberger, P.H.; Eyers, C.E.; Boons, G-J.; Pohl, N.L.B.; Compagnon, I.; Widmalm, G.; Flitsch, S.L. Advancing Solutions to the Carbohydrate Sequencing Challenge J. Am. Chem. Soc. 2019, 141(37), 14463
27. Fallows T.W.; McGrath, A.; Silva, S.; McAdams, S.J.; Marchesi, A; Tuna, F.; Flitsch, S.L.; Tilley, R.; Webb, S. High-throughput Chemical and Chemoenzymatic Approaches to Saccharide-coated Magnetic Nanoparticles for MRI Nanoscale Advances, 2019, 1(9), 3597.
28. Mattey, A.; Birmingham, W.R.; Both, P.; Kress, N.; Huang, K.; van Munster, J.M.; Bulmer, G.; Parmeggiani, F.; Voglmeir, J.; Reyes Martinez, J.E.; Turner, N.J.; Flitsch S.L Selective Oxidation of N-Glycolylneuraminic Acid Using an Engineered Galactose Oxidase Variant ACS Catalysis 2019, 9(9), 8208.
29. Pallister, E.G.; Choo, M.S.F.; Tai, J-N; Leong, D.S.Z.; Tang, W-Q.; Ng, S-K.; Huang, K.; Marchesi, A.; Both, P.; Gray, C.; Rudd, P.M.; Flitsch, S.L.; Nguyen-Khuong, T. Exploiting the Disialyl Galactose Activity of 2,6-Sialyltransferase from Photobacterium damselae to Generate a Highly Sialylated Recombinant -1-Antitrypsin Biochemistry, 2020, 59 (34), 3123
30. Gamayun, I.; O’Keefe, S.; Pick, T.; Klein, M-C.; Nguyen, D.; McKibbin, C.; Piacenti, M.; Williams, H.M.; Flitsch, S.L.; Whitehead, R.C.; Swanton, E.; Helms, V.; High, S.; Zimmermann, R.; Cavalie, A. Eeyarestatin Compounds Selectively Enhance Sec61-Mediated Ca2+ Leakage from the Endoplasmic Reticulum Cell Chemical Biology 2019, 26 (4), 571-583. e6
31. Huang, K.; Flitsch, S.L. Glyco-enzymatic Cascades Get Protection Nature Catalysis 2019, 2 (6), 479. (N&V)
32. Hepworth, L.J.; Flitsch, S.L. Remote Control with Engineered Enzymes Science 2019, 364 (6640), 529. (Perspective)
33. Manning, J.; Tavanti, M.; Porter, J.L.; Kress, N.; De Visser, S.; Turner, N.J.; Flitsch, S.L. Regio- and Enantioselective Chemo-enzymatic C-H Lactonisation of Decanoic acid to (S)--Decalactone Angew. Chemie Int. Ed, 2019, 58 (17), 5668-5671 (highlighted in Synfact: Valorization of a Saturated Fatty Acid to Enantioenriched (S)--Decalactone)
34. Keenan, T.; Mills, R.; Pocock, E.; Budhadeva, D.; Parmeggiani, F.; Flitsch, S.L.; Fascione,M. The Characterisation of a Galactokinase from Streptomyces Coelicolor
Carbohydrate Res. 2019, 472, 132-137
35. Huang, K.; Parmeggiani, F.; Ledru, H.; Hollingsworth, K.; Pons, J.M.; Marchesi, A.; Both, P.; Mattey, A.P.; Pallister, E.; Bulmer, G.S.; van Munster, J.M.; Turnbull, W.B.; Galan, C.; Flitsch, S.L. Enzymatic Synthesis of N-Acetyllactosamine from Lactose Enabled by Recombinant 1-4-Galactosyltransferases Organic & Biomolecular Chemistry 2019, 17, 5920-5924.
36. Cai, Z.P.; Conway, L.P.; Huang, Y.Y.; Wang, W.J.; Laborda, P.; Wang, T.; Lu, A.M.; Yao, H.L.; Huang, K.; Flitsch, S.L.; Liu, L.; Voglmeir, J. Enzymatic Synthesis of Trideuteriated Sialosides, Molecules 2019, 24(7), 1368.
37. Tavanti, M.; Porter, J. L.; Levy, C. W.; Castellanos, J.R.G.; Flitsch, S.L.; Turner, N.J. The Crystal Structure of P450-TT Heme-domain Provides the First Structural Insights Into the Versatile Class VII P450s Biochem.Biophys.Res.Comm. 2018 501 (4) 846-850.
38. Porter, J. L.; Sabatini, S.; Manning, J.; Tavanti, M.; Galman, J.; Turner N. J.; Flitsch, S.L. Cloning, Expression and Characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A Self-sufficient P450 with High Expression and Diverse Substrate Scope Enzyme and Microbial Technology, 2018, 113, 1-8.
39. Song, H. B.; He, M.; Cai, Z. P. Huang, K.; Flitsch, S. L.; Liu, L.; Voglmeir, J. UDP-Glucose 4-Epimerase and b-1-4-Galactosyltransferase from the Oyster Magallana gigas as Valuable Biocatalysts for the production of galactosylated Products International J. Mol. Sci. 2018, 19 (6)
40. Tavanti, M.; Porter, J.L.; Sabatini, S.; Turner, N. J.; Flitsch, S. L. Panel of New Thermostable CYP116B Self-sufficient Cytochrome P450 Monooxygenases that Catalyse C-H Activation with a Diverse Substrate Scope ChemCatChem, 2018, 10 (5) 1042-1051.
41. ST Ahmed, F Parmeggiani, NJ Weise, SL Flitsch, NJ Turner Engineered Ammonia Lyases for the Production of Challenging Electron-rich L-Phenylalanines, ACS Catalysis, 2018, 8 (4) 3129
42. Peter Both, Michel Riese, Christopher J Gray, Kun Huang, Edward G Pallister, Iaroslav Kosov, Louis P Conway, Josef Voglmeir, Sabine L Flitsch Applications of a Highly α2, 6-selective pseudosialidase Glycobiology, 2018, 28 (5) 261
43. Joanne L Porter, Selina Sabatini, Jack Manning, Michele Tavanti, James L Galman, Nicholas J Turner, Sabine L Flitsch Cloning, Expression and Characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A Self-sufficient P450 with High Expression and Diverse Substrate Scope Enzyme and Microbial Technology, 2018, 113, 1
44. Kun Huang, Fabio Parmeggiani, Edward Pallister, Chuen‐Jiuan Huang, Fang‐Fang Liu, Qian Li, William R Birmingham, Peter Both, Baptiste Thomas, Li Liu, Josef Voglmeir, Sabine L Flitsch Characterisation of a Bacterial Galactokinase with High Activity and Broad Substrate Tolerance for Chemoenzymatic Synthesis of 6‐Aminogalactose‐1‐Phosphate and Analogues ChemBioChem, 2018, 19 (4), 388
45. Faye Craven, Joana Silva, Maria Segarra-Maset, Kun Huang, Peter Both, Julie Gough, Sabine L Flitsch, Simon Webb One-pot Sequential Enzymatic Modification of Synthetic Glycolipids in Vesicle Membranes Chemical Communications, 2018, 54 (11) 1347
46. Joanne L Porter, Jack Manning, Selina Sabatini, Michele Tavanti, Nicholas J Turner, Sabine L Flitsch Characterisation of CYP102A25 from B. marmarensis and CYP102A26 from P. halophilus: P450 homologues of BM3 with preference towards hydroxylation of medium chain fatty acids ChemBioChem, 2018, 19 (5) 513
47. Gray, C.; Flitsch S. L. Methods for the High-Resolution Analysis of Glycoconjugates. In ‘Coupling and Decoupling of Diverse Molecular Units in Glycosciences’, 2018, 225-267.
48. Wood, A. J. L; Weise, N. K; Frampton, J. D; Dunstan, M. S; Hollas, M. A; Derrington, S. R; Lloyd, R. C; Quaglia, D; Parmeggiani, F; Leys, D; Turner, N. J and Flitsch, S. L. Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides. Angew. Chemie Int. Ed, 2017. 129 (46), 14690
49. Weissenborn, M. J.; Debecker, D. P; Golyen, S; Linclau, B, Turner, N. J and Flitsch, S. L. Development of a Solid Phase Array Assay for the Screening of Galactose Oxidase Activity and for Fast Identification of Inhibitors. Protein & Peptide Letters, 2017. 24 (8) 742
50. Li, Q.; Huang, Y-Y.; Conway, L.; He, M.; Wei, S.; Huang, K.; Duan, X-C.; Flitsch, S. and Voglmeir, J. Discovery and Biochemical Characterization of a Thermostable Glucose-1-phosphate Nucleotidyltransferase from Thermodesulfatator indicus. Protein & Peptide Letters, 2017. 24 (8) 729
51. Schindler, B.; Barnes, L.; Renois, G.; Gray, C.; Allouche, A-R.; Chambert, S.; Fort, S.; Flitsch, S.; Loison, C. and Compagnon, I. Anomeric Memory of the Glycosidic Bond upon Fragmentation and Its Consequences for Carbohydrate Sequencing. Nature Communications, 2017, 8, 973
52. Hollas, M. A; Webb, S.; Flitsch, S. and Fielding, A. J. A Bifunctional Spin Label for Ligand Recognition on Surfaces. Angew. Chemie Int. Ed., 2017, 56, 9449
53. Gray, C.; Schindler, B.; Migas, L.; Picmanova , M. ; Allouche, A.; Green, A.; Mandal, S.; Motawia, M.; Sánchez-Pérez, R.; Bjarnholt , N. ; Møller, B.; Rijs, A.; Barran, P.; Compagnon, I.; Eyers, C.; Flitsch, S.L. Bottom-up Elucidation of Glycosidic Bond Stereochemistry Analytical Chemistry, 2017, 89 (8), 4540
54. Hepworth, L.; France, S.; Hussain, S.; Both, P.; Turner, N.; Flitsch, S. L. De Novo Enzyme Cascades in Whole Cells for the Synthesis of Chiral Cyclic Amines ACS Catalysis, 2017, 7, 2920
55. Gray, C.; Sánchez-Ruiz, A.; Šardzíková, I.; Ahmed, Y.; Miller, R.; Reyes Martinez, J.; Pallister, E.; Huang, K.; Both, P.; Hartmann, M.; Roberts, H.; Šardzik, R.; Mandal, S.; Turnbull, J.; Eyers, C.; Flitsch, S.L. Label-Free Discovery Array Platform for the Characterization of Glycan Binding Proteins and Glycoproteins Analytical Chemistry, 2017, 89(8), 4444
56. Thomas, B.; Lu, X.; Birmingham, W.R.; Huang, K.; Both, P.; Reyes Martinez, J.E.; Young, R. J.; Davie C. P.; Flitsch, S. L. Application of Biocatalysis to on-DNA Carbohydrate Library Synthesis. ChemBioChem 2017, 18, 1
57. Schindler, B.; Barnes, L.; Gray, C. J.; Chambert, S.; Flitsch, S.L.; Oomens, J.; Daniel, R.; Allouche A. R.; Compagnon, I. IRMPD spectroscopy Sheds New (InfraRed) Light on the Sulfate Pattern of Carbohydrates. J. Phys. Chem. A., 2017, 121(10), 2114
58. van Munster, J. M.; Thomas, B.; Riese, M.; Davis, A. L.; Gray, C. J.; Archer D., B.; Flitsch, S. L. Application of Carbohydrate Arrays Coupled With Mass Spectrometry to Detect Activity of Plant-polysaccharide Degradative Enzymes from the Fungus Aspergillus niger. Scientific Reports, 2017, 43117
59. Klenk, J. M.; Nebel, B. A.; Porter, J. L.; Kulig, J. K.; Hussain, S. A.; Richter, S. M.; Tavanti, M.; Turner, N. J.; Hayes, M. A.; Hauer, B.; Flitsch, S. L. The Self-sufficient P450 RhF Expressed in a Whole Cell System Selectively Catalyses the 5-Hydroxylation of Diclofenac. Biotechnology Journal, 2017, 12(3), 1600520
60. Yan, C.; Parmeggiani, F.; Jones, E. A; Claude, E.; Hussain, S. A; Turner, N. J; Flitsch, S. L; Barran, P. E Real-time Screening of Biocatalysts in Live Bacterial Colonies. J. Am. Chem. Soc., 2017, 139(4), 1408
61. France, S. P; Hepworth, L. J; Turner, N. J.; Flitsch, S. L Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways. ACS Catalysis, 2017, 7(1), 710
62. Ly Y. M, Laborda P, Huang K, Cai P, Wang A. M, Lu C, Doherty C, Liu L, Flitsch S. L and Voglmeir J. Highly efficient and selective biocatalytic production of glucosamine from chitin. Green Chemistry, 2017, 19, 527
63. Ahmed, S. T.; Parmeggiani, F.; Turner, N. J.; Weise, N.J.; Flitsch, S. L. Synthesis of Enantiomerically Pure Ring-Substituted l-Pyridylalanines by Biocatalytic Hydroamination. Org. Lett, 2016, 18(21), 5468
64. Yan, C.; Schmidberger, J. W.; Parmeggiani, F.; Hussain, S. A.; Turner, N. J.; Flitsch, S. L.; Barran, P. Rapid and Sensitive Monitoring of Biocatalytic Reactions Using Ion Mobility Mass Spectrometry. Analyst 2016, 141, 2351
65. Weissenborn, M. J.; Notonier, S.; Lang, S.-L.; Otte, K. B.; Herter, S.; Turner, N. J.; Flitsch, S. L.; Hauer, B. Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. Chemical Communications 2016, 52, 6158
66. Ruscoe, R. E.; Fazakerley, N. J.; Huang, H.; Flitsch, S.; Procter, D. J. Copper‐Catalyzed Double Additions and Radical Cyclization Cascades in the Re‐Engineering of the Antibacterial Pleuromutilin. Chemistry–A European Journal 2016, 22, 116
67. Gray, C.; Thomas, B.; Upton, R.; Migas, L.; Eyers, C.; Barran, P.; Flitsch, S. Applications of ion mobility mass spectrometry for high throughput, high resolution glycan analysis. Biochimica et Biophysica Acta (BBA)-General Subjects 2016, 1860, 1688
68. France, S. P.; Hussain, S.; Hill, A. M.; Hepworth, L. J.; Howard, R. M.; Mulholland, K. R.; Flitsch, S. L.; Turner, N. J. One Pot Cascade Synthesis of Mono-and Di-Substituted Piperidines and Pyrrolidines using Carboxylic Acid Reductase (CAR), ω-Transaminase (ω-TA) and Imine Reductase (IRED) Biocatalysts. ACS Catalysis 2016, 6, 3753-3759.
69. Formisano, N.; Bhalla, N.; Heeran, M.; Martinez, J. R.; Sarkar, A.; Laabei, M.; Jolly, P.; Bowen, C. R.; Taylor, J. T.; Flitsch, S. Inexpensive and fast pathogenic bacteria screening using field- effect transistors. Biosensors and Bioelectronics 2016, 85, 103-109.
70. Eichler, A.; Gricman, Ł.; Herter, S.; Kelly, P. P.; Turner, N. J.; Pleiss, J.; Flitsch, S. L. Enantioselective Benzylic Hydroxylation Catalysed by P450 Monooxygenases: Characterisation of a P450cam Mutant Library and Molecular Modelling. ChemBioChem 2016, 17, 426-432.
71. Castangia, R.; Hudson, S.; Robinson, H.; Flitsch, S.; Thomas-Oates, J.; Routledge, A. Fabrication and Application of Isotopically‐Labelled Gold Arrays for Multiplexed Peptide Analysis. ChemBioChem 2016, 17 (21), 2007-2011.
72. Both, P.; Busch, H.; Kelly, P. P.; Mutti, F. G.; Turner, N. J.; Flitsch, S. L. Whole‐Cell Biocatalysts for Stereoselective C− H Amination Reactions. Angewandte Chemie, International Edition 2016, 55, 1511
73. Voglmeir, J.; Laurent, N.; Flitsch, S. L.; Oelgeschläger, M.; Wilson, I. B. Biological and biochemical properties of two Xenopus laevis N acetylgalactosaminyltransferases with contrasting roles in embryogenesis. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2015, 180, 40
74. Pičmanová, M.; Neilson, E. H.; Motawia, M. S.; Olsen, C. E.; Agerbirk, N.; Gray, C. J.; Flitsch, S.; Meier, S.; Silvestro, D.; Jørgensen, K. A recycling pathway for cyanogenic glycosides evidenced by the comparative metabolic profiling in three cyanogenic plant species. Biochemical Journal 2015, 469, 375
75. Kelly, P. P.; Eichler, A.; Herter, S.; Kranz, D. C.; Turner, N. J.; Flitsch, S. L. Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions. Beilstein Journal of Organic Chemistry 2015, 11, 1713
76. Huang, K.; Wang, M. M.; Kulinich, A.; Yao, H. L.; Ma, H. Y.; Martínez, J. E.; Duan, X. C.; Chen, H.; Cai, Z. P.; Flitsch, S. L. Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila. Carbohydrate Research 2015, 415, 60
77. Ahmed, S. T.; Parmeggiani, F.; Weise, N. J.; Flitsch, S. L.; Turner, N. J. Chemoenzymatic Synthesis of Optically Pure l-and d-Biarylalanines through Biocatalytic Asymmetric Amination and Palladium-Catalyzed Arylation. ACS Catalysis 2015, 5, 5410
78. Noble, G. T.; Craven, F. L.; Segarra-Maset, M. D.; Martínez, J. E. R.; Šardzík, R.; Flitsch, S. L.; Webb, S. J. Sialylation of lactosyl lipids in membrane microdomains by T. cruzi trans-sialidase. Organic & Biomolecular Chemistry 2014, 12, 9272
79. Cai, Z. P.; Hagan, A. K.; Wang, M. M.; Flitsch, S. L.; Liu, L.; Voglmeir, J. 2-Pyridylfuran: a new fluorescent tag for the analysis of carbohydrates. Analytical Chemistry 2014, 86, 5179
80. Both, P.; Green, A.; Gray, C.; Šardzík, R.; Voglmeir, J.; Fontana, C.; Austeri, M.; Rejzek, M.; Richardson, D.; Field, R.; Widmalm, G.; Flitsch, S.L.; Eyers, C.E., Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. Nature Chemistry 2014, 6, 65
81. Serna, S.; Hokke, C. H.; Weissenborn, M.; Flitsch, S.; Martin‐Lomas, M.; Reichardt, N. C. Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays. ChemBioChem 2013, 14, 862-869.
82. O’Reilly, E.; Corbett, M.; Hussain, S.; Kelly, P. P.; Richardson, D.; Flitsch, S. L.; Turner, N. J. Substrate promiscuity of cytochrome P450 RhF. Catalysis Science & Technology 2013, 3, 1490
83. Olkhov, R. V.; Weissenborn, M. J.; Flitsch, S. L.; Shaw, A. M. Glycosylation characterization of human and porcine fibrinogen proteins by lectin-binding biophotonic microarray imaging. Analytical chemistry 2013, 86, 621
84. Martínez, J. E. R.; Šardzík, R.; Voglmeir, J.; Flitsch, S. L. Enzymatic synthesis of colorimetric substrates to determine α-2, 3-and α-2, 6-specific neuraminidase activity. RSC Advances 2013, 3, 21335
85. Lopez-Castejon, G.; Luheshi, N. M.; Compan, V.; High, S.; Whitehead, R. C.; Flitsch, S.; Kirov, A.; Prudovsky, I.; Swanton, E.; Brough, D. Deubiquitinasesregulate the activity of caspase-1 and interleukin-1β secretion via assembly of the inflammasome. Journal of Biological Chemistry 2013, 288, 2721
86. Gray, C. J.; Weissenborn, M. J.; Eyers, C. E.; Flitsch, S. L. Enzymatic reactions on immobilised substrates. Chemical Society Reviews 2013, 42, 6378
87. Weissenborn, M. J.; Wehner, J. W.; Gray, C. J.; Šardzík, R.; Eyers, C. E.; Lindhorst, T. K.; Flitsch, S. L. Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS. Beilstein Journal of Organic Chemistry 2012, 8, 753
88. Weissenborn, M. J.; Castangia, R.; Wehner, J. W.; Šardzík, R.; Lindhorst, T. K.; Flitsch, S.L Oxo-ester mediated native chemical ligation on microarrays: an efficient and chemoselective coupling methodology. Chemical Communications 2012, 48, 4444
89. Wehner, J. W.; Weissenborn, M. J.; Hartmann, M.; Gray, C. J.; Šardzík, R.; Eyers, C. E.; Flitsch, S. L.; Lindhorst, T. K. Dual purpose S-trityl-linkers for glycoarray fabrication on both polystyrene and gold. Organic & biomolecular chemistry 2012, 10, 8919
90. Seeliger, D.; Zapater, C.; Krenc, D.; Haddoub, R.; Flitsch, S.; Beitz, E.; Cerdà, J.; de Groot, B. L. Discovery of novel human aquaporin-1 blockers.
ACS chemical biology 2012, 8, 249
91. Šardzík, R.; Green, A. P.; Laurent, N.; Both, P.; Fontana, C.; Voglmeir, J.; Weissenborn, M. J.; Haddoub, R.; Grassi, P.; Haslam, S. M.; Flitsch S.L. Chemoenzymatic synthesis of O- mannosylpeptides in solution and on solid phase. Journal of the American Chemical Society 2012, 134, 4521
92. O’Reilly, E.; Aitken, S. J.; Grogan, G.; Kelly, P. P.; Turner, N. J.; Flitsch, S. L. Regio-and stereoselective oxidation of unactivated C–H bonds with Rhodococcus rhodochrous. Beilstein Journal of Organic Chemistry 2012, 8, 496
93. Noble, G. T.; Craven, F. L.; Voglmeir, J.; Sardzik, R.; Flitsch, S. L.; Webb, S. J. Accelerated enzymatic galactosylation of N-acetylglucosaminolipids in lipid microdomains. Journal of the American Chemical Society 2012, 134, 13010
94. McKibbin, C.; Mares, A.; Piacenti, M.; Williams, H.; Roboti, P.; Puumalainen, M.; Callan, A. C.; Lesiak-Mieczkowska, K.; Linder, S.; Harant, H.: High, S.; Flitsch, S.L.; Whitehead, R.C.; Swanton, E. Inhibition of protein translocation at the endoplasmic reticulum promotes activation of the unfolded protein response. Biochemical Journal 2012, 442, 639-648.
95. Laurent, N.; Haddoub, R.; Voglmeir, J.; Flitsch, S. L. MALDI-ToF MS analysis of glycosyltransferase activities on gold surface arrays. Carbohydrate Microarrays: Methods and Protocols 2012, 269
96. Castangia, R.; Austeri, M.; Flitsch, S. L. Enzymatic Amine Acyl Exchange in Peptides on Gold Surfaces. Angewandte Chemie International Edition 2012, 51, 13016
97. Voglmeir, J.; Kaloo, S.; Laurent, N.; Meloni, M. M.; Bohlmann, L.; Wilson, I. B.; Flitsch, S. L. Biochemical correlation of activity of the α-dystroglycan-modifying glycosyltransferase POMGnT1 with mutations in muscle-eye-brain disease. Biochemical Journal 2011, 436, 447
98. Šardzík, R.; Sharma, R.; Kaloo, S.; Voglmeir, J.; Crocker, P. R.; Flitsch, S. L. Chemoenzymatic synthesis of sialooligosaccharides on arrays for studies of cell surface adhesion. Chemical Communications 2011, 47, 5425
99. Šardzík, R.; Both, P.; Flitsch, S. L. Post-translational modifications: S-linked sugars lost and found. Nature Chemical Biology 2011, 7, 69
100. Robin, A.; Köhler, V.; Jones, A.; Ali, A.; Kelly, P. P.; O’Reilly, E.; Turner, N. J.; Flitsch, S.L. Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope. Beilstein Journal of Organic Chemistry 2011, 7, 1494
101. Rannes, J. B.; Ioannou, A.; Willies, S. C.; Grogan, G.; Behrens, C.; Flitsch, S. L.; Turner, N. J. Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution. Journal of the American Chemical Society 2011, 133, 8436
102. O’Reilly, E.; Köhler, V.; Flitsch, S. L.; Turner, N. J. Cytochromes P450 as useful biocatalysts: addressing the limitations. Chemical Communications 2011, 47, 2490
103. Ioannou, A.; Cini, E.; Timofte, R. S.; Flitsch, S. L.; Turner, N. J.; Linclau, B. Heavily fluorinated carbohydrates as enzyme substrates: oxidation of tetrafluorinated galactose by galactose oxidase. Chemical Communications 2011, 47, 11228-11230.
104. Gotor, V.; Flitsch, S. Increasing the diversity of biocatalytic reactions. Current Opinion in Chemical Biology 2011, 15, 185-186.
105. Alissandratos, A.; Baudendistel, N.; Hauer, B.; Baldenius, K.; Flitsch, S.; Halling, P. Biocompatible functionalisation of starch Chemical Communications 2011, 47, 683-685.
106. Aletrari, M.-O.; McKibbin, C.; Williams, H.; Pawar, V.; Pietroni, P.; Lord, J. M.; Flitsch, S. L.; Whitehead, R.; Swanton, E.; High, S. Eeyarestatin 1 interferes with both retrograde and anterograde intracellular trafficking pathways. PloS one 2011, 6, e22713.
107. Voglmeir, J.; Šardzík, R.; Weissenborn, M. J.; Flitsch, S. L. Enzymatic glycosylations on arrays. Omics: a journal of integrative biology 2010, 14, 437
108. Tey, L. H.; Loveridge, E. J.; Swanwick, R. S.; Flitsch, S. L.; Allemann, R. K. Highly site‐ selective stability increases by glycosylation of dihydrofolate reductase. FEBS journal 2010, 277, 2171
109. Šardzík, R.; Noble, G. T.; Weissenborn, M. J.; Martin, A.; Webb, S. J.; Flitsch, S. L. Preparation of aminoethyl glycosides for glycoconjugation. Beilstein journal of organic chemistry 2010, 6, 699
110. Sabbadin, F.; Hyde, R.; Robin, A.; Hilgarth, E. M.; Delenne, M.; Flitsch, S.; Turner, N.; Grogan, G.; Bruce, N. C. LICRED: A Versatile Drop‐In Vector for Rapid Generation of Redox‐Self‐ Sufficient Cytochrome P450s. ChemBioChem 2010, 11, 987
111. Liem, K. P.; Noble, G. T.; Flitsch, S. L.; Webb, S. J. The effect of multivalent binding on the lateral phase separation of adhesive lipids. Faraday Discussions 2010, 145, 219
112. Alissandratos, A.; Baudendistel, N.; Flitsch, S. L.; Hauer, B.; Halling, P. J. Lipase- catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC. BMC Biotechnology 2010, 10, 1.
113. Robin, A.; Roberts, G. A.; Kisch, J.; Sabbadin, F.; Grogan, G.; Bruce, N.; Turner, N. J.; Flitsch, S. L. Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme. Chemical Communications 2009, 2478
114. Noble, G. T.; Flitsch, S. L.; Liem, K. P.; Webb, S. J. Assessing the cluster glycoside effect during the binding of concanavalin A to mannosylated artificial lipid rafts. Organic & Biomolecular Chemistry 2009, 7, 5245
115. Haddoub, R.; Rützler, M.; Robin, A.; Flitsch, S. L. Design, synthesis and assaying of potential aquaporin inhibitors. In Aquaporins; Springer Berlin Heidelberg, 2009; pp 385-402.
116. Haddoub, R.; Laurent, N.; Meloni, M. M.; Flitsch, S. L. Straightforward Synthesis of 2- Acetamido-2-deoxy-β-d-glucopyranosyl Esters under Microwave Conditions. Synlett 2009, 3328
117. Haddoub, R.; Dauner, M.; Stefanowicz, F. A.; Barattini, V.; Laurent, N.; Flitsch, S. L. Enzymatic synthesis of peptides on a solid support Organic & Biomolecular Chemistry 2009, 7, 665
118. Cross, B. C.; McKibbin, C.; Callan, A. C.; Roboti, P.; Piacenti, M.; Rabu, C.; Wilson, C. M.; Whitehead, R.; Flitsch, S. L.; Pool, M. R. Eeyarestatin I inhibits Sec61-mediated protein translocation at the endoplasmic reticulum. Journal of cell science 2009, 122, 4393
119. Bejugam, M.; Flitsch, S. L. Enzyme Cleavable Linker Units. Linker Strategies in Solid-Phase Organic Synthesis 2009, 221
120. Basso, A.; Sinigoi, L.; Gardossi, L.; Flitsch, S. Effect of microwave radiation on enzymatic and chemical peptide bond synthesis on solid phase. International Journal of Peptides 2009, 2009: 362482
121. Bahamontes-Rosa, N.; Bucher, K.; Held, J.; Robin, A.; Hoffmann, W.; Flitsch, S.; Kremsner, P.; Kun, J. In vivo anti-malarial effect of the ß-amino alcohol 1t on Plasmodium berghei. Parasitology research 2009, 104, 1459
122. Zhi, Z. L.; Laurent, N.; Powell, A. K.; Karamanska, R.; Fais, M.; Voglmeir, J.; Wright, A.; Blackburn, J. M.; Crocker, P. R.; Russell, D. A. A versatile gold surface approach for fabrication and interrogation of glycoarrays. ChemBioChem 2008, 9, 1568
123. Laurent, N.; Voglmeir, J.; Wright, A.; Blackburn, J.; Pham, N. T.; Wong, S. C.; Gaskell, S. J.; Flitsch, S. L. Enzymatic glycosylation of peptide arrays on gold surfaces. ChemBioChem 2008, 9, 883
124. Laurent, N.; Voglmeir, J.; Flitsch, S. L. Glycoarrays—tools for determining protein– carbohydrate interactions and glycoenzyme specificity. Chemical Communications 2008, 4400
125. Laurent, N.; Haddoub, R.; Voglmeir, J.; Wong, S. C.; Gaskell, S. J.; Flitsch, S. L. SPOT Synthesis of Peptide Arrays on Self‐Assembled Monolayers and their Evaluation as Enzyme Substrates. ChemBioChem 2008, 9, 2592
126. Laurent, N.; Haddoub, R.; Flitsch, S. L. Enzyme catalysis on solid surfaces. Trends in Biotechnology 2008, 26, 328
127. Karamanska, R.; Clarke, J.; Blixt, O.; MacRae, J. I.; Zhang, J. Q.; Crocker, P. R.; Laurent, N.; Wright, A.; Flitsch, S. L.; Russell, D. A. Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays. Glycoconjugate Journal 2008, 25, 69
128. Deere, J.; De Oliveira, R. F.; Tomaszewski, B.; Millar, S.; Lalaouni, A.; Solares, L. F.; Flitsch, S. L.; Halling, P. J. Kinetics of enzyme attack on substrates covalently attached to solid surfaces: influence of spacer chain length, immobilized substrate surface concentration and surface charge. Langmuir 2008, 24, 11762-11769.
129. Bahamontes-Rosa, N.; Robin, A.; Ambrosio, A.; Messias-Reason, I.; Beitz, E.; Flitsch, S.; Kun, J. Monoquaternary ammonium derivatives inhibit growth of protozoan parasites. Parasitology international 2008, 57, 132
130. Robin, A.; Brown, F.; Bahamontes-Rosa, N.; Wu, B.; Beitz, E.; Kun, J. F.; Flitsch, S. L. Microwave-assisted ring-opening of epoxides: a general route to the synthesis of 1-aminopropan-2-ols with anti malaria parasite activities. Journal of Medicinal Chemistry 2007, 50, 4243
131. Flitsch, S. Enzyme Assays. High‐throughput Screening, Genetic Selection and Fingerprinting. Herausgegeben von Jean‐Louis Reymond. (book review) Angewandte Chemie 2007, 119, 4508
132. Deere, J.; McConnell, G.; Lalaouni, A.; Maltman, B. A.; Flitsch, S. L.; Halling, P. J. Real‐ Time Imaging of Protease Action on Substrates Covalently Immobilised to Polymer Supports. Advanced Synthesis & Catalysis 2007, 349, 1321
133. Rawsterne, R. E.; Gough, J. E.; Rutten, F. J.; Pham, N. T.; Poon, W. C.; Flitsch, S. L.; Maltman, B.; Alexander, M. R.; Ulijn, R. V. Controlling protein retention on enzyme‐responsive surfaces. Surface and Interface Analysis 2006, 38, 1505
134. Detmers, F. J.; De Groot, B. L.; Müller, E. M.; Hinton, A.; Konings, I. B.; Sze, M.; Flitsch, S. L.; Grubmüller, H.; Deen, P. M. Quaternary Ammonium Compounds as Water Channel Blockers – specificity, potency, and site of action. Journal of Biological Chemistry 2006, 281, 14207
135. Çelik, A.; Roberts, G. A.; White, J. H.; Chapman, S. K.; Turner, N. J.; Flitsch, S. L. Probing the substrate specificity of the catalytically self-sufficient cytochrome P450 RhF from a Rhodococcus sp. Chemical communications 2006, 4492
136. Swanwick, R. S.; Daines, A. M.; Tey, L. H.; Flitsch, S. L.; Allemann, R. K. Increased Thermal Stability of Site‐Selectively Glycosylated Dihydrofolate Reductase. ChemBioChem 2005, 6, 1338
137. Swanwick, R. S.; Daines, A. M.; Flitsch, S. L.; Allemann, R. K. Synthesis of homogenous site-selectively glycosylated proteins. Organic & Biomolecular Chemistry 2005, 3, 572
138. Maltman, B. A.; Bejugam, M.; Flitsch, S. L. Enzyme-cleavable linkers for peptide and glycopeptide synthesis. Organic & Biomolecular Chemistry 2005, 3, 2505
139. Hunter, D. J.; Roberts, G. A.; Ost, T. W.; White, J. H.; Müller, S.; Turner, N. J.; Flitsch, S. L.; Chapman, S. K. Analysis of the domain properties of the novel cytochrome P450 RhF. FEBS Letters 2005, 579, 2215
140. Halling, P. J.; Ulijn, R. V.; Flitsch, S. L. Understanding enzyme action on immobilised substrates. Current Opinion in Biotechnology 2005, 16, 385
141. Flitsch, S. L. Synthetic chemistry: Glycosylation with a twist. Nature 2005, 437, 201
142. Çelik, A.; Flitsch, S. L.; Turner, N. J. Efficient terpene hydroxylation catalysts based upon P450 enzymes derived from actinomycetes. Organic & Biomolecular Chemistry 2005, 3, 2930
143. Bejugam, M.; Maltman, B. A.; Flitsch, S. L. Synthesis of N-linked glycopeptides on solid support and their evaluation as protease substrates. Tetrahedron: Asymmetry 2005, 16, 21
144. Basso, A.; Maltman, B. A.; Flitsch, S. L.; Margetts, G.; Brazendale, I.; Ebert, C.; Linda, P.; Verdelli, S.; Gardossi, L. Optimized polymer–enzyme electrostatic interactions significantly improve penicillin G amidase efficiency in charged PEGA polymers. Tetrahedron 2005, 61, 971
145. Speight, R. E.; Hancock, F. E.; Winkel, C.; Bevinakatti, H. S.; Sarkar, M.; Flitsch, S. L.; Turner, N. J. Rapid identification of cytochrome P450 cam variants by in vivo screening of active site libraries. Tetrahedron: Asymmetry 2004, 15, 2829
146. Roberts, G. A.; Grogan, G.; Turner, N. J.; Flitsch, S. L. Nucleotide Sequence of a Portion of the Camphor-degrading Gene Cluster from Rhodococcus sp. NCIMB 9784. DNA Sequence 2004, 15, 96
147. Moore, B. D.; Stevenson, L.; Watt, A.; Flitsch, S.; Turner, N. J.; Cassidy, C.; Graham, D. Rapid and ultra-sensitive determination of enzyme activities using surface-enhanced resonance Raman scattering. Nature Biotechnology 2004, 22, 1133
148. Lins, R. J.; Flitsch, S. L.; Turner, N. J.; Irving, E.; Brown, S. A. Generation of a dynamic combinatorial library using sialic acid aldolase and in situ screening against wheat germ agglutinin. Tetrahedron 2004, 60, 771
149. Doezé, R. H.; Maltman, B. A.; Egan, C. L.; Ulijn, R. V.; Flitsch, S. L. Profiling primary protease specificity by peptide synthesis on a solid support. Angewandte Chemie International Edition 2004, 43, 3138
150. Daines, A. M.; Maltman, B. A.; Flitsch, S. L. Synthesis and modifications of carbohydrates, using biotransformations. Current Opinion in Chemical Biology 2004, 8, 106
151. Bejugam, M.; Flitsch, S. L. An efficient synthetic route to glycoamino acid building blocks for glycopeptide synthesis. Organic letters 2004, 6, 4001
152. Basso, A.; Ulijn, R. V.; Flitsch, S. L.; Margetts, G.; Brazendale, I.; Ebert, C.; De Martin, L.; Linda, P.; Verdelli, S.; Gardossi, L. Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support. Tetrahedron 2004, 60, 589
153. Ulijn, R. V.; Flitsch, S. L.; Humphrey, C. E.; Easson, M. A.; Turner, N. J.
Lipase- Catalyzed Kinetic Resolution on Solid-Phase Via a” Capture and Release” Strategy. Journal of the American Chemical Society 2003, 125, 13952
154. Ulijn, R. V.; Brazendale, I.; Margetts, G.; Flitsch, S. L.; McConnell, G.; Girkin, J.; Halling, P. J. Two-photon microscopy to spatially resolve and quantify fluorophores in single-bead chemistry Journal of Combinatorial Chemistry 2003, 5, 215
155. Ulijn, R. V.; Bisek, N.; Halling, P. J.; Flitsch, S. L. Understanding protease catalysed solid phase peptide synthesis. Organic & Biomolecular Chemistry 2003, 1, 1277
156. Ulijn, R. V.; Bisek, N.; Flitsch, S. L. Enzymatic optical resolution via acylation– hydrolysis on a solid support. Organic & Biomolecular Chemistry 2003, 1, 621
157. Sprung, I.; Carmès, L.; Watt, G. M.; Flitsch, S. L. Synthesis of novel acceptor substrates for the dolichyl phosphate mannose synthase from yeast. ChemBioChem 2003, 4, 319
158. Roberts, G. A.; Celik, A.; Hunter, D. J.; Ost, T. W.; White, J. H.; Chapman, S. K.; Turner, N. J.; Flitsch, S. L. A self-sufficient cytochrome P450 with a primary structural organization that includes a flavin domain and a [2Fe-2S] redox center. Journal of Biological Chemistry 2003, 278, 48914
159. Flitsch, S. L.; Ulijn, R. V. Sugars tied to the spot. Nature 2003, 421, 219
160. Bosma, A. Y.; Ulijn, R. V.; McConnell, G.; Girkin, J.; Halling, P. J.; Flitsch, S. L. Using two photon microscopy to quantify enzymatic reaction rates on polymer beads. Chemical Communications 2003, 22(22), 2790
161. Basso, A.; De Martin, L.; Gardossi, L.; Margetts, G.; Brazendale, I.; Bosma, A. Y.; Ulijn, V.; Flitsch, S. L. Improved biotransformations on charged PEGA supports. Chemical Communications 2003, 1296
162. Basso, A.; De Martin, L.; Ebert, C.; Linda, P.; Gardossi, L.; Ulijn, R. V.; Flitsch, S. L. Organically modified xerogels as supports for solid-phase chemistry. Tetrahedron Letters 2003, 44, 6083
163. Ulijn, R. V.; Baragaña, B.; Halling, P. J.; Flitsch, S. L. Protease-catalyzed peptide synthesis on solid support. Journal of the American Chemical Society 2002, 124, 10988
164. Sprung, I.; Ziegler, A.; Flitsch, S. L. Enzymatic synthesis of β-mannosyl phosphates on solid support. Chemical Communications 2002, 2676
165. Roberts, G. A.; Grogan, G.; Greter, A.; Flitsch, S. L.; Turner, N. J. Identification of a New Class of Cytochrome P450 from a Rhodococcus sp. Journal of Bacteriology 2002, 184, 3898
166. Pohl, N.; Cammack, R.; Hall, D.; Projan, S.; Halliwell, C.; Cass, T.; Stewart, J. D.; Kieran, A. L.; Sanders, J. K.; Flitsch, S. Chemical biology. Current Opinion in Chemical Biology 2002, 6, 405
167. Macmillan, D.; Daines, A. M.; Bayrhuber, M.; Flitsch, S. L. Solid-phase synthesis of thioether-linked glycopeptide mimics for application to glycoprotein semisynthesis. Organic Letters 2002, 4, 1467
168. Lins, R. J.; Flitsch, S. L.; Turner, N. J.; Irving, E.; Brown, S. A. Enzymatic generation and in situ screening of a dynamic combinatorial library of sialic acid analogues. Angewandte Chemie International Edition 2002, 41, 3405
169. Grogan, G.; Roberts, G.; Parsons, S.; Turner, N.; Flitsch, S. P450camr, a cytochrome P450 catalysing the stereospecific 6-endo-hydroxylation of (1R)-(+)-camphor.Applied Microbiology and Biotechnology 2002, 59, 449
170. Flitsch, S; Grogan, G.; Ashcroft, D. Oxygenation of C-H and C-C bonds Catalysis in Organic Synthesis: A Comprehensive Handbook, Second Edition 2002, 1065
171. Flitsch, S.; Grogan, G. Baeyer‐Villiger Oxidations. Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook, Second Edition 2002, 1202
172. Matthews, L.; Tewari, M.; Pohl, N.; Cammack, R.; Hall, D.; Projan, S.; Stewart, J. D.; Roberts, S. L.; Sanders, J. K.; Flitsch, S. Chemical biology. Current Opinion in Chemical Biology 2001, 5, 229
173. Macmillan, D.; Bill, R. M.; Sage, K. A.; Fern, D.; Flitsch, S. L. Selective in vitro glycosylation of recombinant proteins: semi-synthesis of novel homogeneous glycoforms of human erythropoietin. Chemistry & Biology 2001, 8, 133
174. Grogan, G.; Roberts, G. A.; Bougioukou, D.; Turner, N. J.; Flitsch, S. L. The desymmetrization of bicyclic -diketones by an enzymatic retro-claisen reaction. A new reaction of the crotonase superfamily. Journal of Biological Chemistry 2001, 276, 12565
175. Grogan, G.; Graf, J.; Jones, A.; Parsons, S.; Turner, N. J.; Flitsch, S. L. An Asymmetric Enzyme‐Catalyzed Retro‐Claisen Reaction for the Desymmetrization of Cyclic β‐Diketones. Angewandte Chemie International Edition 2001, 40, 1111
176. Watt, G. M.; Flitsch, S. L.; Fey, S.; Elling, L.; Kragl, U. The preparation of deoxy derivatives of mannose-1-phosphate and their substrate specificity towards recombinant GDP-mannose pyrophosphorylase from Salmonella enterica, group B. Tetrahedron: Asymmetry 2000, 11, 621
177. Lellouch, A.; Watt, G.; Geremia, R.; Flitsch, S. Phytanyl-pyrophosphate-linked substrate for a bacterial α-mannosyltransferase. Biochemical and Biophysical Research Communications 2000, 272, 290
178. Flitsch, S. L.; Watt, G. M. Enzymes in carbohydrate chemistry: formation of glycosidic linkages. Biotechnology Set, Second Edition 2000, 243
179. Flitsch, S. L. Chemical and enzymatic synthesis of glycopolymers. Current Opinion in Chemical Biology 2000, 4, 619
180. Taylor, P.; Anderson, V.; Dowden, J.; Flitsch, S. L.; Turner, N. J.; Loughran, K.; Walkinshaw, M. D. Novel mechanism of inhibition of elastase by β-lactams is defined by two inhibitor crystal complexes. Journal of Biological Chemistry 1999, 274, 24901
181. Revers, L.; Bill, R. M.; Wilson, I. B.; Watt, G. M.; Flitsch, S. L. Development of recombinant, immobilised β-1, 4-mannosyltransferase for use as an efficient tool in the chemoenzymatic synthesis of N-linked oligosaccharides. Biochimica et Biophysica Acta (BBA)-General Subjects 1999, 1428, 88
182. Flitsch, S. L.; Aitken, S. J.; Chow, C. S.-Y.; Grogan, G.; Staines, A. Biohydroxylation reactions catalyzed by enzymes and whole-cell systems. Bioorganic Chemistry 1999, 27, 81
183. Flitsch, S. Carbohydrates in Drug Design Edited by ZJ Witczak and KA Nieforth. Glycoconjugate Journal 1998, 15, 949
184. Böhm, G.; Dowden, J.; Rice, D. C.; Burgess, I.; Pilard, J.-F.; Guilbert, B.; Haxton, A.; Hunter, R. C.; Turner, N. J.; Flitsch, S. L. A novel linker for the attachment of alcohols to solid supports. Tetrahedron Letters 1998, 39, 3819
185. Aitken, S.; Chow, C.-Y.; Turner, N.; Flitsch, S. Biohydroxylations of Cbz-protected alkyl substituted piperidines by Beauveria bassiana ATCC 7159. Journal of the Chemical Society, Perkin Transactions 1 1998, 3365
186. Watt, G. M.; Revers, L.; Webberley, M. C.; Wilson, I. B.; Flitsch, S. L. The chemoenzymatic synthesis of the core trisaccharide of N-linked oligosaccharides using a recombinant β-mannosyltransferase. Carbohydrate Research 1997, 305, 533
187. Watt, G. M.; Revers, L.; Webberley, M. C.; Wilson, I. B.; Flitsch, S. L. Efficient Enzymatic Synthesis of the Core Trisaccharide of N‐Glycans with a Recombinant β‐ Mannosyltransferase. Angewandte Chemie International Edition in English 1997, 36, 2354
188. Watt, G. M.; Lowden, P. A.; Flitsch, S. L. Enzyme-catalyzed formation of glycosidic linkages. Current Opinion in Structural Biology 1997, 7, 652
189. Routledge, A.; Stock, H. T.; Flitsch, S. L.; Turner, N. J. New fluoride-labile linkers for solid-phase organic synthesis. Tetrahedron Letters 1997, 38, 8287
190. Riley, M. L.; Wallace, B.; Flitsch, S. L.; Booth, P. J. Slow α helix formation during folding of a membrane protein. Biochemistry 1997, 36, 192
191. Proud, A. D.; Prodger, J. C.; Flitsch, S. L. Development of a protecting group for sulfate esters. Tetrahedron Letters 1997, 38, 7243
192. Booth, P. J.; Riley, M. L.; Flitsch, S. L.; Templer, R. H.; Farooq, A.; Curran, A. R.; Chadborn, N.; Wright, P. Evidence that bilayer bending rigidity affects membrane protein folding. Biochemistry 1997, 36, 197-203.
193. Bill, R. M.; Flitsch, S. L.; Bicknell, R. COS-1 cell expression and one-step affinity protein purification and activity of epitope-tagged human erythropoietin and of site-directed mutants. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology 1997, 1340, 13
194. Booth, P. J.; Farooq, A.; Flitsch, S. L. Retinal binding during folding and assembly of the membrane protein bacteriorhodopsin. Biochemistry 1996, 35, 5902
195. Bill, R. M.; Flitsch, S. L. Chemical and biological approaches to glycoprotein synthesis. Chemistry & Biology 1996, 3, 145
196. Wilson, I.; Webberley, M.; Revers, L.; Flitsch, S. Dolichol is not a necessary moiety for lipid-linked oligosaccharide substrates of the mannosyltransferases involved in in vitro N-linked-oligosaccharide assembly. Biochemical Journal 1995, 310, 909
197. Booth, P. J.; Flitsch, S. L.; Stern, L. J.; Greenhalgh, D. A.; Kim, P. S.; Khorana, H. G. Intermediates in the folding of the membrane protein bacteriorhodopsin. Nature Structural &Molecular Biology 1995, 2, 139
198. Bill, R. M.; Winter, P. C.; McHale, C. M.; Hodges, V. M.; Elder, G. E.; Caley, J.; Flitsch, L.; Bicknell, R.; Lappin, T. R. Expression and mutagenesis of recombinant human and murine erythropoietins in Escherichia coli. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression 1995, 1261, 35
199. Revers, L.; Wilson, I.; Webberley, M.; Flitsch, S. The potential dolichol recognition sequence of β-1, 4-mannosyltransferase is not required for enzymic activity using phytanyl- pyrophosphoryl-α-N, N’-diacetylchitobioside as acceptor. Biochemical Journal 1994, 299, 23
200. Guilbert, B.; Flitsch, S. L. A short chemo-enzymic route to glycosphingolipids using soluble glycosyl transferases. Journal of the Chemical Society, Perkin Transactions 1 1994, 1181
201. Guilbert, B.; Davis, N. J.; Pearce, M.; Aplin, R. T.; Flitsch, S. L. Dibutylstannylene acetals: Useful intermediates for the regioselective sulfation of glycosides. Tetrahedron: Asymmetry 1994, 5, 2163
202. Guilbert, B.; Davis, N. J.; Flitsch, S. L. Regioselective sulfation of disaccharides using dibutylstannylene acetals. Tetrahedron Letters 1994, 35, 6563
203. Flitsch, S. L.; Goodridge, D. M.; Guilbert, B.; Revers, L.; Webberley, M. C.; Wilson, I. B. The chemoenzymatic synthesis of neoglycolipids and lipid-linked oligosaccharides using glycosyltransferases. Bioorganic & Medicinal chemistry 1994, 2, 1243
204. Davis, N. J.; Flitsch, S. L. Chemical synthesis of disaccharides which are partial structures of the glycosaminoglycan heparan sulfate. Journal of the Chemical Society, Perkin Transactions 1 1994, 359
205. Wilson, I.; Taylor, J.; Webberley, M.; Turner, N.; Flitsch, S. Phytanyl and mono- branched lipid substrates for dolichyl phosphate mannose synthetase and β mannosyltransferase. Glycoconjugate Journal 1993, 10, 265
206. Wilson, I.; Taylor, J.; Webberley, M.; Turner, N.; Flitsch, S. A novel mono-branched lipid phosphate acts as a substrate for dolichyl phosphate mannose synthetase. Biochemical Journal 1993, 295, 195
207. Guilbert, B.; Flitsch, S. A novel chemo-enzymatic synthesis of a neoglycolipid. Glycoconjugate Journal 1993, 10, 325-326.
208. Davis, N. J.; Flitsch, S. L. Selective oxidation of monosaccharide derivatives to uronic acids. Tetrahedron Letters 1993, 34, 1181
209. Guilbert, B.; Khan, T. H.; Flitsch, S. L. Chemoenzymatic synthesis of a glycosphingolipid. J. Chem. Soc., Chem. Commun. 1992, 1526
210. Flitsch, S. L.; Pinches, H. L.; Taylor, J. P.; Turner, N. J. Chemo-enzymatic synthesis of a lipid-linked core trisaccharide of N-linked glycoproteins. Journal of the Chemical Society, Perkin Transactions 1 1992, 2087
211. Flitsch, S. L.; Taylor, J. P.; Turner, N. J. Synthesis of a novel acceptor substrate for a mannosyl transferase. J. Chem. Soc., Chem. Commun. 1991, 380
212. Flitsch, S. L.; Taylor, J. P.; Turner, N. J. Chemo-enzymatic synthesis of a β-mannosyl- containing trisaccharide. Journal of the Chemical Society, Chemical Communications 1991, 382
213. Davis, N. J.; Flitsch, S. L. A novel method for the specific glycosylation of proteins. Tetrahedron Letters 1991, 32, 6793
214. Coe, D.; Flitsch, S.; Hilpert, H.; Liebster, M.; Roberts, S.; Turner, N. An Improved Method for the Synthesis of Highly Substituted Alkyl Phosphates Including Nucleoside 5′‐Phosphates. ChemInform 1990, 21.
215. Flitsch, S. L.; Khorana, H. G. Structural studies on transmembrane proteins. 1. Model study using bacteriorhodopsin mutants containing single cysteine residues. Biochemistry 1989, 28, 7800
216. Altenbach, C.; Flitsch, S. L.; Khorana, H. G.; Hubbell, W. L. Structural studies on transmembrane proteins. 2. Spin labeling of bacteriorhodopsin mutants at unique cysteines. Biochemistry 1989, 28, 7806
217. Khorana, H.; Braiman, M.; Chao, B.; Doi, T.; Flitsch, S.; Gillesgonzales, M.; Hackett, N.; Karnik, S.; Mogi, T.; Nassal, M. Site-specific mutagenesis in structure-function studies of bacteriorhodopsin. CHEMICA SCRIPTA 1987, 27, 137-147.
218. Baldwin, J. E.; Adlington, R. M.; Flitsch, S. L.; Ting, H.-H.; Turner, N. J. Penicillin biosynthesis: the origin of hydroxy groups in β-lactams derived from unsaturated substrates. Journal of the Chemical Society, Chemical Communications 1986, 1305
219. Baldwin, J. E.; Adlington, R. M.; Basak, A.; Flitsch, S. L.; Petursson, S.; Turner, N. J.; Ting, H.-H. Enzymatic synthesis of a new type of penicillin.
Journal of the Chemical Society, Chemical Communications 1986, 975-976.
220. Baldwin, J. E.; Adlington, R. M.; Basak, A.; Flitsch, S. L.; Forrest, A. K.; Ting, H.-H. Penicillin biosynthesis: structure–reactivity profile of unsaturated substrates for isopenicillin N synthetase. Journal of the Chemical Society, Chemical Communications 1986, 273-275.
221. Baldwin, J. E.; Abraham, E. P.; Adlington, R. M.; Bahadur, G. A.; Chakravarti, B.; Domayne-Hayman, B. P.; Field, L. D.; Flitsch, S. L.; Jayatilake, G. S.; Špakovaskis, A. Penicillin biosynthesis: active site mapping with aminoadipoylcysteinylvaline variants. Journal of the Chemical Society, Chemical Communications 1984, 1225-122.